- 期刊
Reduction of Classical Swine Fever Virus-Specific Cell Proliferative Response of Porcine Peripheral Blood Mononuclear Cells by Porcine Circovirus Type 2
豬第二型環狀病毒降低豬瘟病毒特異性周邊血液單核細胞的增殖反應
摘要
兔化豬瘟（lapinized Philippines Coronel；LPC）疫苗的保護效力，已證實會受到豬第二型環狀病（porcine circovirus type 2；PCV2）感染而干擾。為了瞭解PCV2干擾LPC疫苗的機制，本研究將建立豬瘟病（classical swine fever virus；CSFV）特異性周邊血液單核細胞（peripheral blood mononuclear cells；PBMC）增殖反應的檢測方法，並利用此法評估PCV2對CSFV特異性細胞性免疫反應的影響。檢測結果，強毒ALD病毒株誘發的PBMC增殖反應顯著較LPC疫苗病毒株強，而不同的ALD病毒感染劑量所誘發的PBMC增殖反應也不一，3個感染量（multiplicity of infection；MOI）的ALD感染組顯著較0.1至1 MOI ALD感染組低。進一步測試PCV2感染確實可顯著降低CSFV特異性的PBMC增殖反應與PBMC的CD25表現量，但PCV2基因體內的CpG-ODN則不具有此抑制作用；另外，發現IL-2、IL-4、IL-10及TNF-α於各組間並無顯著差異。綜合上述結果顯示，PCV2感染確實可降低CSFV特異性的PBMC增殖反應，且此PBMC增殖反應的干擾可能與CD25的表現有關。
並列摘要
Porcine circovirus type 2 (PCV2) infection reducing the efficacy of Lapinized Philippines Coronel (LPC) vaccine of classical swine fever virus (CSFV) has been demonstrated in the previous study. In order to investigate the possible mechanisms of PCV2-derived interference on the CSFV-specific cell-mediated immune response, an ex vivo model of CSFV-specific cell proliferative response of peripheral blood mononuclear cells (PBMCs) from PCV2-carrier pigs was established. The ALD, a virulent CSFV strain, induced a significantly higher CSFV-specific cell proliferative response than the attenuated LPC strain did. The ALD strain at a dose of 0.1-1 multiplicity of infection (MOI) induced a significantly higher CSFV-specific cell proliferative response than that at 3 MOI did. Pre-inoculated PCV2 significantly reduced the levels of CSFV-specific cell proliferative response and CD25 expression in PBMCs than that of ALD alone. The PCV2-derived interference was not associated with the CpG-ODNs of PCV2 genome neither the cytokine levels of IL-2, IL-4, IL-10, and TNF-α in the supernatant of PBMC cultures. These results indicated that PCV2 could reduce CSFV-specific cell proliferative response in PBMCs partially through the reduction of CD25 expression in stimulated PBMCs.
延伸閱讀
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