Viral Distribution and Depress of Body Weight of a Duck Picornavirus in Experimentally Infected Peking Ducklings and Serological Survey among Poultry Species in Taiwan

鴨小核醣核酸病毒於實驗性感染北京鴨之病毒分布與抑制增重及台灣禽類血清學調查

周崇熙(Chung-Hsi Chou) ； 丁彥文(Yen-Wen Ting) ； 曾俊憲(Chun-Hsien Tseng) ； 施雨華(Yu-Hua Shih) ； 潘銘正(Ming-Jen Pan) ； 蔡向榮(Hsiang-Jung Tsai)

《臺灣獸醫學雜誌》 39卷2期 (2013/06) Pp. 73-80

摘要

Duck hepatitis (DH) mainly caused by Duck hepatitis A virus serotype 1 (DHAV-1) and serotype 2 (DHAV-2) belonging to Avihepatovirus of Picornaviridae is a high mortality disease in ducklings. An untypical DH-pathogen, duck picornavirus (DPV) strain Tw90A, isolated in 1990 in Taiwan belongs to Sapelovirus of Picornaviridae was studied herein. In the experiment 1, none mortality or any obvious clinical symptoms were observed in the ducklings receiving 10^(6.25) TCID50 strain Tw90A intraperitoneally, but the body weight gain within the infection group was significantly lower than the control group (p＜0.05). In the experiment 2, by using the RT-PCR and the Nested-RT-PCR, the viral genome was detected constantly in cerebrum, cerebellum, trachea, cardiac muscle, liver, spleen, kidney, and rectum in the inoculated ducklings from 8-hour to 21-day post-inoculation. In addition, the DPV-antibody field seroprevalence study demonstrated a 33.0% positive rate in duck which was significantly higher (p＜0.01) than those of goose (7.1%) and chicken (1.5%). Meanwhile, the unexpected high DPV-sero-positive rate of pigeon (22.5%) needed a further study clarifying a possible DPV transmission role. Comparing to sero-positive rates of DHAV-1 (62.3%) and DHAV-2 (1.1%), it is suggested that DHAV-1 is the dominant DH etiology in duck population in Taiwan.

關鍵字

鴨病毒性肝炎 ； 鴨小核醣核酸病毒 ； 人工感染 ； 血清盛行率

並列摘要

Duck hepatitis (DH) mainly caused by Duck hepatitis A virus serotype 1 (DHAV-1) and serotype 2 (DHAV-2) belonging to Avihepatovirus of Picornaviridae is a high mortality disease in ducklings. An untypical DH-pathogen, duck picornavirus (DPV) strain Tw90A, isolated in 1990 in Taiwan belongs to Sapelovirus of Picornaviridae was studied herein. In the experiment 1, none mortality or any obvious clinical symptoms were observed in the ducklings receiving 10^(6.25) TCID50 strain Tw90A intraperitoneally, but the body weight gain within the infection group was significantly lower than the control group (p＜0.05). In the experiment 2, by using the RT-PCR and the Nested-RT-PCR, the viral genome was detected constantly in cerebrum, cerebellum, trachea, cardiac muscle, liver, spleen, kidney, and rectum in the inoculated ducklings from 8-hour to 21-day post-inoculation. In addition, the DPV-antibody field seroprevalence study demonstrated a 33.0% positive rate in duck which was significantly higher (p＜0.01) than those of goose (7.1%) and chicken (1.5%). Meanwhile, the unexpected high DPV-sero-positive rate of pigeon (22.5%) needed a further study clarifying a possible DPV transmission role. Comparing to sero-positive rates of DHAV-1 (62.3%) and DHAV-2 (1.1%), it is suggested that DHAV-1 is the dominant DH etiology in duck population in Taiwan.

並列關鍵字

Duck hepatitis ； Duck picornavirus ； Experimental infection ； Seroprevalence