探討Chitinase 3-like 1作為犬乳腺腫瘤預後評估因子之適當性

Investigating the Potential of Chitinase 3-like 1 as the Prognostic Factor of Canine Mammary Gland Tumors

江欣蒨(Hsin-Chien Chiang) ； 莊添富(Tien-Fu Chuang) ； 廖泰慶(Albert Tai-Ching Liao) ； 朱瑞民(Rea-Min Chu) ； 林辰栖(Chen-Si Lin)

《臺灣獸醫學雜誌》 39卷3期 (2013/09) Pp. 158-165

摘要

犬乳腺腫瘤為母犬最好發的腫瘤之一，且其中約有一半被確診為惡性。由於目前並無有效的分子診斷標記作為腫瘤良惡性、惡性腫瘤分級、以及預後的判定之用，因此本研究旨在探討類幾丁質酶（Chitinase 3-like 1, CHI3L1）作為犬乳腺腫瘤分級及預測患犬預後之可行性。藉由分析臨床犬乳腺腫瘤檢體，我們檢測包括CHI3L1、MERTK以及APOE共3種基因在腫瘤組織中的表現情形。結果顯示：在惡性犬乳腺腫瘤中，受測的3種基因其表現量均顯著的高於良性腫瘤。進一步探討這些基因表現在惡性腫瘤的WHO臨床分級中的情況時，其結果顯示僅有CHI3L1具有區分腫瘤分級的效果：其在Stage I 的表現量顯著高於臨床上最為惡性的Stage IV/V；且在惡性乳腺腫瘤的罹癌犬中，CHI3L1表現量越高，患犬的存活時間越長。綜合前述，我們認為CHI3L1具有判別犬乳腺腫瘤良惡性、區分惡性分級、以及預測患犬存活時間的潛能，故值得進一步的分析與研究。

關鍵字

犬乳腺腫瘤 ； 酪氨酸激酶 ； 載脂蛋白E ； 腫瘤診斷

並列摘要

Canine mammary gland tumors (cMGTs) are among the most common neoplastic processes in female dogs and approximately 50% of them are malignant. To date, there are no efficient biomarkers for the diagnosis of cMGT malignancy, malignant tumor grading, and prognosis. In this study, we examined the gene expression of chitinase 3-like 1 (CHI3L1), tyrosine kinase Mer (MERTK), and apolipoprotein E (APOE) in cMGT clinical specimens to investigate if these genes could be used as biomarkers for cMGT diagnosis. The results indicated that all the three genes were over-expressed in malignant cMGT. However, among the malignant cMGT samples, only CHI3L1 was highly expressed in Stage I malignant cMGT tissues compared to Stage IV/V cases. Furthermore, cMGT patients with higher CHI3L1 expression lived longer than those expressing lower amounts of CHI3L1. Taken together, although more cases are needed to confirm the results, we have provided the hints that CHI3L1 may be a potential biomarker for cMGT diagnosis.

並列關鍵字

Chitinase 3-like 1 ； canine mammary gland tumors ； CHI3L1 ； MERTK ； APOE ； tumor diagnosis