溫控影響錦鯉感染錦鯉疱疹病毒病原與抗體之表現

Water Temperature Regulate the Performance of Koi Herpesvirus Infection and Antibody Response in Koi

劉尚杰(Shang-Chieh Liu) ； 張本恆(Pen-Heng Chang) ； 楊雨樵(Yu-Chiao Yang) ； 涂堅(Chien Tu) ； 陳媺玫(Meei-Mei Chen)

《臺灣獸醫學雜誌》 39卷3期 (2013/09) Pp. 174-181

摘要

錦鯉疱疹病毒（koi herpesvirus, KHV）被歸類在鯉魚疱疹病毒第三型（Cyprinid herpesvirus 3, CyHV-3），為感染錦鯉及鯉魚並造成高死亡率之高度傳染性疾病病原。本研究模擬魚隻感染KHV的情形，將20隻鯉魚置於KHV好發溫度下（23℃），分成兩組，以不同病毒劑量進行浸泡攻毒，並在魚隻進入急性期開始死亡後將水溫提高到好發溫度範圍之外（32℃），使病毒無法繼續複製造成魚隻死亡。感染KHV錦鯉魚病原以聚合酶連鎖反應（Polymerase chain reaction, PCR）和巢式PCR （Nested-PCR）進行檢測，並以血清中和試驗和間接酵素免疫連結反應持續監測魚隻體內抗KHV的抗體。結果顯示，魚隻死亡時間集中在攻毒後十到二十天；血清抗體力價約於攻毒後十天開始出現，並在攻毒後二十到三十天到達高值，持續到攻毒後七十天左右才開始下降。病原檢測方面，當魚隻處於KHV好發溫度範圍外時，PCR幾乎無法檢測到KHV病原，但Nested-PCR仍有陽性檢出，即這些存活的魚隻可能為潛伏感染或持續感染。

關鍵字

錦鯉疱疹病毒 ； 帶原魚 ； 檢測方法

並列摘要

Koi herpesvirus (KHV), recently designated Cyprinid herpesvirus 3 (CyHV-3), is the etiological agent of an emerging and fatal disease in common carp (Cyprinius carpio carpio) and koi (Cyprinus carpio koi). In this study we tried to imitate the detectable pathogen and antibody produced condition when carp were infected with KHV. Twenty Koi carps exposed to the virus at permissive temperature, and then transferred to non-permissive temperature (32℃) in disease acute phase. PCR and Nested-PCR methods were used to identify KHV virus, and neutralization test and ELISA were used to monitor serum antibody. Results showed that death occurred at 10-20 dpi. Antibody titer began to rise at 10dpi, each a peak about 20-30 dpi, and maintained at plateau until 70 dpi. When koi became latent infection under non-permissive temperature, KHV can't identify by PCR method, while Nested-PCR still had positive result.

並列關鍵字

Koi Herpesvirus ； carrier ； detection methods