The purpose of this study is to compare the differences of the speed, frequency and length of strides of an elite Japanese runner, Sumie Inagak, and the Taiwanese runner, Shu-Jung Chiu, in Soochow University Ultra Marathon 24hr Race. We video-record the two runners throughout 24 hours in the competition and used a comprehensive motion analysis software, Kwon3D, with the time and spatial (3D) parameters to collect stride frequency and stride length hourly. The statistical analyses were analyzed by SPSS 12.0 and Microsoft Excel. We found that both runners reduced their running speed significantly while the time increased. No significant difference of the stride frequency has been found between two runners. However, the Japanese runner has greater stride length than the Taiwanese runner and this difference might influence their running performance.