滲透壓誘導水稻癒合組織植株再生過程中過氧化酵素之變化

Changes in Isoperoxidases in Rice (Oryza sativa L.) Callus during Shoot Regeneration Induced by Osmotic Stress

黃文理(Wen-Lii Huang) ； 劉麗飛(Li-Fei Liu)

《作物、環境與生物資訊》 3卷1期 (2006/03) Pp. 1-8

https://doi.org/10.30061/CEB.200603.0001

摘要

本研究主要探討水稻癒合組織植株再生過程中過氧化酵素活性與同功酵素之變化。結果顯示具高分化能力TN5-M6癒合組織在植株再生過程中，不論是可溶性(soluble)與細胞壁結合(wall-bound)過氧化酵素活性均明顯高於不具分化能力的TN5-M0，其中wall-bound過氧化酵素活性呈現雙高峰曲線，且其活性變化與癒合組織綠點及小苗形成有密切相關。此外，作者建立以等電點電泳分析過氧化酵素同功酵素圖譜技術，發現在移到分化培養基後第一天，具高分化能力TN5-M6癒合組織中pI 4.5與pI 5.1同功酵素即明顯表現，而在第3天綠點形成後，pI 4.7與pI 6.2同功酵素表現愈來愈明顯，顯示癒合組織不同發育階段有不同過氧化酵素表現，極適合作為水稻癒合組織植株再生之標誌酵素。

關鍵字

水稻 ； 植株再生 ； 過氧化酵素 ； 同功酵素 ； 等電點電泳

並列摘要

Changes of peroxidase activity and isozyme pattern in rice callus during shoot regeneration induced by osmotic stress were investigated. The results showed that shoot regeneration in rice callus was accompanied by an increase in specific activity either soluble or wall-bound peroxidase. Besides, the peroxidase isozyme patterns were separated using isoelectric focusing (IEF) electrophoresis. It showed that the pI 4.5 and pI 5.1 were predominant isozymes in TN5-M6, the highly regenerative callus, at the first day on regeneration medium. The pI 4.7 and pI 6.2 isozymes were also significant before plantlet formation. Results indicate that different peroxidase isozymes are expressed at callus and shoot regeneration stage and can be easily monitored by IEF analysis. These isozymes are useful markers for shoot regeneration in rice callus.

並列關鍵字

Oryza sativa ； Shoot regeneration ； Peroxidase ； Isozymes ； Isoelectric focusing electrophoresis