Tagging Rice Drought-related QTL with SSR DNA Markers

利用微衛星分子標誌偵測水稻耐旱基因QTL

林孟輝(Meng-Huei Lin) ； 林家偉(Chia-Wei Lin) ； 陳若竹(Jo-Chu Chen) ； 林育宗(Yu-Chung Lin) ； 鄭舒允(Shu-Yun Cheng) ； 劉宗華(Tzung-Hua Liu) ； 詹富智(Fuh-Jyh Jan) ； 吳詩都(Shu-Tu Wu) ； 曾富生(Fu-Sheng Thseng) ； 古新梅(Hsin-Mei Ku)

《作物、環境與生物資訊》 4卷1期 (2007/03) Pp. 65-76

https://doi.org/10.30061/CEB.200703.0007

摘要

乾旱是限制臺灣水稻生育的主要因子之一，因此本研究以525個水稻微衛星DNA標誌(SSR)篩選水稻臺中189號（日本型，對乾旱敏感品種）、密陽23號（印度型，耐旱品種）以及二者雜交後代F2間多形性，以探討水稻耐旱基因位置。結果有121個SSR分子標誌能分辨多型性，並偵測出四個與耐旱有關之SSR分子標誌，分別為dis4.1（位於第4條染色體）、lr8.1（位於第8條染色體）、lr10.1（位於第10條染色體）、及lr12.1（位於第12條染色體）。另外，本研究亦偵測出影響水稻農藝性狀之QTL，如QTL RM136 (y6.1, chromosome 6)、RM537 (y4.1, chromosome 4)、RM5443 (y1.1, chromosome 1)、RM3231 (y8.1, chromosome 8)及RM3(y6.2, chromosome 6)等5個QTL與產量有關聯，因此利用這些與耐旱有緊密關聯之SSR DNA標誌，未來將可進行早期世代選拔及縮短育種時程。

關鍵字

水分逆境 ； 耐旱 ； 水稻

並列摘要

Drought is one of the major limited factors that affect the growth of rice in Taiwan. The chromosomal regions associated with drought tolerance in rice using SSR mapping system were detected in this study. A F2 mapping population was generated from the cross between Taichung 189 (japonica type), a local drought susceptible rice, and Milyang 23 (indica type), a drought tolerant line. A total of 525 SSR DNA markers were used to screen polymorphisms between parents. There were 121 SSR DNA markers showed polymorphisms and were mapped on the F2 population. A total of 4 SSR DNA markers, dis4.1, lr8.1, lr10.1, and lr12.1 on chromosomes 4, 8, 10, and 12 were associated with drought tolerance. Other QTL affecting agronomically important traits were also reported. Five QTL RM136 (y6.1, chromosome 6), RM537 (y4.1, chromosome 4), RM5443 (y1.1, chromosome 1), RM3231 (y8.1, chromosome 8), and RM3 (y6.2, chromosome 6) were associated with yield. Applying these SSR DNA markers that are closely linked with drought tolerance will facilitate early selection of drought tolerant lines and shorten breeding period.

並列關鍵字

Water stress ； Drought tolerant ； Rice Oryza sativa L.