- 期刊
受逆境調控之水稻醛糖還原酶基因之表現
Expression Profiling of Stress-responsive Aldose Reductase Genes from Rice
摘要
醛糖還原酶(aldose reductase)普遍存在動植物體內，被認為與細胞解毒作用、糖醇生合成及逆境抗性有關。本研究利用生物資訊分析，針對水稻基因體主要資料庫進行搜尋比對，發現在水稻基因組中至少可比對出5個aldose reductase基因(OsARs)，為一多基因族，分別位於第1、第5及第10條染色體上，可編碼出305 到375個不等長度之胺基酸序列，與已發表aldose reductase 基因序列比對，可區分為三群。設計專一性引子，選殖出5個OsARs基因，進一步利用懸浮培養系統，發現5個OsARs基因中，OsAR5基因表現明顯受ABA與sorbitol誘導，屬於逆境誘導基因；相反地，OsAR1與OsAR4基因表現則受ABA、NaCl及sorbitol抑制，屬於逆境抑制表現基因，而OsAR2與OsAR3基因則為持續表現型基因。OsARs基因於水稻幼苗系統之表現分析，結果則與懸浮培養系統不完全一致，其中OsAR4與OsAR5明顯呈現根部專一性與ABA誘導表現，OsAR3則主要在地上部表現。本文為首篇觀察有關水稻OsARs基因表現之試驗研究報告，未來將進一步深入分析水稻植株發育過程與不同部位中OsARs基因之表現，並釐清OsAR基因之生理功能及與逆境耐受性之關係。
並列摘要
Aldose reductase is widely distributed in animal and plant tissues and has been considered related to function in cellular detoxification, sugar alcohol biosynthesis, and stress tolerance. In the present study, genome-wide database search indicating at least five different aldose reductase genes (OsARs) were located at chromosomes 1, 5 and 10 of rice genome, respectively. OsARs belong to a multigene family and the encoded proteins have various lengths, ranging from 305 to 375 amino acids. Results of phylogenetic analysis showed that these proteins may be classified into three main clusters. Genes cloning and expression patterns under stress treatments were furtherper formed with suspension culture system. Results indicated that OsAR5 mRNA level was significantly increased in suspension cells treated with ABA and sorbitol, whereas no significant changes were observed for OsAR2 and OsAR3isogenes under ABA and sorbitol treatments. On the other hand, the mRNA levels of OsAR1 and OsAR4 were decreased by ABA, salt, and sorbitol treatments. Results suggest that OsARs genes might closely relate to stress response. To our knowledge, this is the first report that regards OsARs gene expression pattern under stresses in rice. Further studies are necessary to unravel the possible roles of OsARs genes on stress resistance and sugar metabolism.
延伸閱讀
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