成茶品種快速分子鑑定技術之研究及應用

Studies on the Fast Molecular Identification Technologies of Made Tea (Camellia sinensis) Varieties and Their Applications

林亞平(Ya-Ping Lin) ； 胡智益(Chih-Yi Hu) ； 蔡右任(You-Zenn Tsai) ； 林順福(Shun-Fu Lin)

《作物、環境與生物資訊》 7卷1期 (2010/03) Pp. 37-51

https://doi.org/10.30061/CEB.201003.0003

摘要

為了有效鑑定國產及進口成茶品種，本研究進行研發快速、有效和價格低廉之成茶分子鑑定系統。本研究篩選出3條ISSRDNA引子及PAL2引子對，這些引子共可產生12條電泳條帶，可做為鑑別臺灣茶樹栽培品種之核心分子標誌。另外，由5條ISSR引子及DFR3引子對所產生之12個分子標誌，亦可做為品種鑑定之備用工具。同時將不同引子混合進行PCR反應常出現分子標誌不穩定之現象，然而將不同引子所得PCR產物混合，則未發現不穩定現象。將兩個茶葉品種DNA依不同比例（10、20、30、40及50%）混合，進行葉綠體DNA分子標誌檢測，結果顯示利用DNA序列變異均可有效檢測出10%以上比例之混合品種樣品。比賽茶樣品分析發現部分非當地常見之品種參與比賽，而進口及國內市場所取得之茶葉樣品均發現不知品種名稱或可能為混合品種之茶葉樣品。

關鍵字

成茶 ； 品種鑑定 ； 分子標誌 ； 核心標誌

並列摘要

The objective of this study was to develop a fast, effective and inexpensive molecular system to distinguish varieties of made tea cultivated in Taiwan or imported from other countries. Three ISSR and PAL2 DNA primers amplifying 12 core markers were developed for identifying tea varieties cultivated in Taiwan. And additional 12 markers amplified by 5 ISSR and DFR3 primers were prepared for variety discrimination. Unstable electrophoresis results were found in the multiplex PCR reaction, but not found in the mixture of PCR products. Various compositions (10, 20, 30, 40 and 50%) of DNA mixtures from different varieties were analyzed, and the results indicated that single variety with more than 10% in the mixture could be effectively identified with chloroplast DNA sequences. Some mixed-variety samples were detected in the specimen for local Tea Contest. Some commercial products collected from imported or local markets were identified as unknown varieties or mixtures of different varieties.

並列關鍵字

Made tea ； Variety identification ； Molecular marker ； Core marker