異戊烯轉移酶（ipt）基因轉殖青花菜與近緣亞種作物雜交潛力及後代之性狀探討

基因轉殖作物之基因流佈可能造成轉殖基因雜交後代植株競爭優勢，為生態安全評估主要議題之ㄧ。本研究異戊烯轉移酶（isopentenyl transferase; ipt）基因轉殖青花菜103轉殖系與7種近緣亞種作物作為花粉接受親進行田間自然雜交潛力評估。田間自然雜交率範圍在0-9%,屬於種間雜交之小白菜及小油菜皆未發現雜交現象，證實種內基因流佈存在。為進一步探討轉殖株雜交後代植株之競爭能力，乃於溫室利用基因轉殖ipt青花菜與自交系104（CK）為花粉貢獻親，分別與近緣亞種作物進行人工雜交。整體而言，F1後代植株性狀以103轉殖系雜交F1後代株高較高，多數性狀仍在104（CK）雜交F1後代、近緣亞種及104（CK）範圍內，但每果莢種子數顯著高於104（CK）雜交F1後代及雜交親本，顯示基因轉殖ipt青花菜雜交近緣亞種F1後代仍具有相當的雜交優勢。

關鍵字

雜交親和力；生長勢；雜種優勢；花粉活性；生物安全評估

並列摘要

Genetically modified crops may cause transgene distribution of hybrid plants. Issues about the ecological security assessment become more and more important. In this study, broccoli (Brassica oleracea var. italica) transformed with the ipt (isopentenyl transferase) gene, designated as line 103 was used to investigate hybridization extent by pollination with seven closely related varieties. The range of fertility rates followed by nature pollination were 0-9%, while no hybridization was found for 'Pakchoi' and 'Rapa' as pollen recipients. Results showed that transgenic DNA insertion did not alter potential risk for outcross among broccoli varieties. In order to study the competitive advantage of F_1 progenies bred from transgenic ipt broccoli crossing with four closely related varieties, transgenic line 103 and 104 (CK) inbred line were used as pollen donor and hybridized with related varieties by artificial pollination. Results of transgenic ipt F_1 showed that plant height was higher than that of 104 hybrid F_1. Seed numbers per pod of transgenic ipt F_1 were higher than those of 104 hybrid F_1, parents and 104 (CK). Results indicated that F_1 progeny from transgenic broccoli hybrid exhibited the phenomenon of heterosis.

並列關鍵字

Cross compatibility ； Growth vigor ； Heterosis ； Pollen viability ； Biosafety assessment

國際替代計量

異戊烯轉移酶（ipt）基因轉殖青花菜與近緣亞種作物雜交潛力及後代之性狀探討

全文下載

主題瀏覽