The Inducible Transposon System for Rice Functional Genomics

可誘導轉位子應用於水稻功能基因體學

常玉強(Yuh-Chyang Charng) ； 吳紀德(Gideon Wu) ； 謝嘉珊(Chia-Shan Hsieh) ； 莊函寧(Han-Ning Chuan) ； 黃紀穎(Ji-Ying Huang) ； 葉凌君(Ling-Chun Yeh) ； 謝亞雄(Ya-Hsiung Shieh) ； 杜鎮(Jenn Tu)

《Botanical Studies》 48卷1期 (2007/01) Pp. 1-11

摘要

Ac/Ds轉位子為研究植物功能基因體學之重要工具。本研究以Ac轉位子為基礎建構「可誘導轉位子」系統，並應用於水稻功能基因體學。可誘導轉位子INAc以PR-1a啟動子驅動Ac轉位酶，在水稻癒合組織中可受水楊酸誘導而轉位，且水楊酸劑量影響轉位效率；分析跳出之轉位子側翼序列顯示轉位子跳入染色體新位置後可遺傳至下一代，證明「可誘導轉位子」系統可應用於水稻功能基因體學研究。

關鍵字

Ac轉位酶 ； 可誘導啟動子 ； 水楊酸 ； 基因釣取

並列摘要

The one-component inducible transposon system for rice functional genomic studies was assessed. In contrast to the native Ac transposon, INAc contains the transposase gene under the control of the inducible promoter (PR-1a) from tobacco. To examine whether INAc can be used in cereals, the behavior of INAc was analyzed with transgenic rice plants containing a single copy of the INAc element. Treatment of rice calli with salicylic acid induced transposition of INAc in somatic tissue, and the transposition efficiency of INAc was dose dependent. Furthermore, a high throughput method for detection of new transposed INAc was developed. Analyzing the flanking sequences of the transposed INAc indicated the independent insertions. Given the fact that a number of different types of Ac/Ds vectors have been already examined in rice, the importance of a ”controlled” transposon system to yield knockout mutants or new transgenic plants was discussed.

並列關鍵字

Ac transposase ； Inducible promoter ； Salicylic acid ； Transposon tagging