Generic Limits of Pyrinae: Insights from NuclearRibosomal DNA Sequences

基於核基因序列探討蘋果亞科的屬間界限

李慶豔(Qing-Yan Li) ； 郭微(Wei Guo) ； 廖文波(Wen-Bo Liao) ； James A. Macklin ； 李建華(Jian-Hua Li)

《Botanical Studies》 53卷1期 (2012/01) Pp. 151-164

摘要

Pyrinae 亞族（原蘋果亞科）為單系類群，約具1,000 個種。本亞族包括了許多著名的水果：如蘋果、梨、榅桲、枇杷、野櫻莓、唐棣。本亞族的分類一直很混亂，特別是對蘋果屬、花楸屬和石楠屬的分類一直存在爭議。本次研究共包括180 個ITS 序列，代表了本亞族內所有的屬，本文即利用ITS 分子序列分析來研究Pyrinae 亞族的屬間關係。ITS 序列分析結果顯示，以下24 屬得到確認，即：Amelanchier、Aria（ 包括 Micromeles）、Aronia、Chaenomeles、Chamaemespilus、Chamaemeles、Cormus、Cotoneaster、Crataegus、Cydonia、Dichotomanthes、Eriobotrya、Hesperomeles、Malacomeles、Malus（包括 Chloromeles, Docynia, Docyniopsis 和 Eriolobus）、Mespilus、Osteomeles、Peraphyllum、Pourthiaea、Pseudocydonia、Pyrus、Rhaphiolepis、Sorbus 和 Torminalis。但石楠屬和火棘屬則顯示為多起源，包括了Heteromeles 和Stranvaesia，所以它們與亞族內其它屬的關係尚未解決。研究結果支持唐棣屬與Malacomeles、Peraphyllum 具有較近的親緣關係，山楂屬和歐楂屬具有較近的親緣關係，並第一次確定了南美的Hesperomeles 與Crataegus-Mespilus 具較近的親緣關係。

關鍵字

蘋果亞科 ； Pyrinae ； nrDNA ITS ； 屬間關係 ； Hesperomeles

並列摘要

The subtribe Pyrinae, formerly the Maloideae, is a monophyletic group of about 1,000 species that includes well known fruit crops such as apple (Malus), pear (Pyrus), quince (Cydonia), loquat (Eriobotrya), chokeberry (Aronia), and serviceberry (Amelanchier). Generic limits have been fluid in Pyrinae, especially in Malus, Sorbus and Photinia. This study evaluated the generic limits of 180 samples of multiple species or accessions from each of the traditional genera using sequences of the nrDNA ITS region. The ITS data recognized 24 genera, including Amelanchier, Aria (including Micromeles), Aronia, Chaenomeles, Chamaemespilus, Chamaemeles, Cormus, Cotoneaster, Crataegus, Cydonia, Dichotomanthes, Eriobotrya, Hesperomeles, Malacomeles, Malus (including Chloromeles, Docynia, Docyniopsis, and Eriolobus), Mespilus, Osteomeles, Peraphyllum, Pourthiaea, Pseudocydonia, Pyrus, Rhaphiolepis, Sorbus, and Torminalis. However, both Photinia and Pyracantha are polyphyletic. Photinia is separated into different clades, one of which contains species of Heteromeles and Stranvaesia. Asian species of Pyracantha do not form a clade with P. coccinea of southern Europe and Iran. Our results support the close relationship of Amelanchier, Malacomeles, and Peraphyllum, and of Crataegus and Mespilus, and for the first time recognize the sister relationship of the South American genus Hesperomeles with the Crataegus-Mespilus clade.

並列關鍵字

Generic limits ； Hesperomeles ； Maloideae ； nrDNA ITS Pyrinae