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- OpenAccess
Ultraviolet Radiation-absorbing Mycosporine-like Amino Acids in Cyanobacterium Aulosira fertilissima: Environmental Perspective and Characterization
並列摘要
The aim of this study is to screen cyanobacterial strains for the high yield of Mycosporine-like amino acids and further the effect of various physicochemical conditions were also observed for its highest yield. Cyanobacteria are one of the most primitive organisms capable of MAAs synthesis. The UV screening compounds MAAs are usually accumulated intracellularly in cyanobacteria. Among the 18 strains, Aulosira fertilissima, showed the presence of highest amount of MAAs hence selected for the exposure of various physicochemical conditions (pH, light quality, UV-light, photoperiod and temperature). MAAs content was highly increased by UV exposure. 20 min exposure of UV-light induces three times highest amount of MAAs as compared to control. In the presence of pH stress also the content of MAAs was approximately three times higher than control while light quality and temperature had very little effect on MAA concentration. The highperformance liquid chromatographic analysis of water-soluble compounds reveals the biosynthesis of two MAAs, porphyra-334 (8_max = 334 nm) and shinorine (8_max = 334 nm), with retention times of 3.5 and 2.3 min, respectively. Spectrophotometric analysis also showed absorption maxima at 334 nm.
並列關鍵字
Absorption spectrum ； Aulosira fertilissima ； HPLC ； MAAs ； physicochemical stress ； Porphyra-334 ； shinorine延伸閱讀
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