- 期刊
- OpenAccess
Hypoglycemic Activity of Aqueous and Ethylacetate Leaf and Stem Bark Extracts of ＂Pappea capensis＂ in Alloxan-Induced Diabetic BALB/c Mice
並列摘要
The present study was performed to determine the optimal dose of alloxan monohydrate required to induce diabetes in male BALB/c mice and investigate in vivo hypoglycemic activity of aqueous and ethylacetate leaf and stem bark extracts of ＂Pappea capensis＂ L in alloxanized diabetic BALB/c mice. In addition, the proximate composition of ＂P. capensis＂ powder was investigated. The seven groups used in determining the optimal alloxan dose to induce diabetes included the normal mice intraperitoneally administered with a single dose of 0.1 mL physiological saline and doses of 50.0, 77.6, 120.4, 186.9, 290.0 and 480 mg/kg body weight in 0.1 mL of physiological saline. Blood glucose levels were determined at 0, 24 and 48 h using a glucometer. The hypoglycemic activity of aqueous and ethylacetate extracts was studied in the normal and diabetic mice orally administered with 0.1 mL physiological saline; diabetic mice orally administered with 0.075 mg glibenclamide, 1.25, 2.5 and 5 mg extract all in 0.1 mL physiological saline. Blood glucose levels were determined at 0, 2, 4, 6 and 24 h, respectively. The proximate composition of ＂P. capensis＂ powder was estimated using standard procedures. Results show that a single dose of alloxan at 186.9 mg/kg body weight administered to 3-5 weeks old mice induced stable diabetes in 48 h; oral administration of ethylacetate leaf and stem bark extracts at 100 and 200 mg/kg body weight induced hypoglycemic activity in a dose independent manner which was similar to that of glibenclamide at 3 mg/kg body weight from the second to the 24^(th) h. Total ash and lipid were higher while the crude protein and carbohydrate were lower in leaves compared to the stem barks. In conclusion, ＂P. capensis＂ is a nutritious plant whose ethylacetate extracts possess in vivo hypoglycemic activity.
延伸閱讀
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