- 期刊
- OpenAccess
Association of Sodium Induced High Blood Pressure with Up-Regulation of Inducible Nitric Oxide Synthase (iNOS) Expression and Inhibition of Trimethylation of H3K27 on Its Promoter Region
並列摘要
Nitric oxide (NO), a simple molecule, playing very diverse roles in the regulation of the cardiovascular, immunologic and nervous system, is formed from L-arginine by converting it to L-citrulline via nitric oxide synthase enzymes (NOS) including endothelial NOS (eNOS), neuronal NOS (nNOS) and inducible NOS (iNOS). We previously reported that long term inhibition of iNOS by intravenous administration of aminogunidine (AG) led to an aggravation of hypertension induced by high sodium chloride (NaCl) loading. Also, high blood pressure induced by high NaCl loading is associated with higher NO production in SD rats. The goal of the present study was to clarify whether the change of blood pressure alter iNOS gene expression as well as epigenetic modification of iNOS gene promoter region. Up-regulation of iNOS mRNA and protein were observed in renal medullary tissue of high sodium infused rats. Furthermore, chromatin immunoprecipitation assay evidenced the inhibition of recruitment of methyltransferase enhancer of Zeste homology 2 (EZH2) to, and trimethylation of histone 3 (H3) at lysine 27 (K27) of iNOS gene promoter region. We concluded that blood pressure is a regulator of iNOS gene expression through alteration of histone covalent modification of its genomic DNA.
被引用紀錄
延伸閱讀
- Feng, A. N., Yin, W. H., Young, M. S., & Lin, M. W. (2005). Association between 894G>T Polymorphism in the Endothelial Nitric Oxide Synthase Gene and Circadian Variation of Blood Pressure in Patients with Essential Hypertension. Acta Cardiologica Sinica, 21(3), 137-145. https://www.airitilibrary.com/Article/Detail?DocID=10116842-200509-21-3-137-145-a
- Halloran, M., Parakh, S., & Atkin, J. D. (2013). The Role of S-Nitrosylation and S-Glutathionylation of Protein Disulphide Isomerase in Protein Misfolding and Neurodegeneration. International Journal of Cell Biology, 2013(), 518-532. https://doi.org/10.1155/2013/797914
- Liu, Y. C., Chan, J. Y. H., & Tsai, Y. C. (2009). Influence of Propofol on Blood Pressure Spectrum in Sepsis and the Role of Inducible Nitric Oxide Synthase. Acta Anaesthesiologica Taiwanica, 47(2), 62-70. https://www.airitilibrary.com/Article/Detail?DocID=02541319-200906-200906260046-200906260046-62-70
- 張美娟（2004）。The inhibitory effects of thalidomide and honokiol on inflammation-induced inducible nitric oxide synthase and cyclooxygenase-2 expression in renal cells〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2004.01964
- Hsu, M. F. (2010). Chemical Probe-Based Approach to Delineate the Underlying Mechanism for Nitric Oxide-Mediated Inactivation of Protein Tyrosine Phosphatases in Regulation of Insulin Signaling [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.00693