Recently, industries of business consultancy and services and software are substantially growing, so they need a large amount of talents having e-Business knowledge. In order to satisfy the demand, we have to popularize the e-Business knowledge. This study focuses on how to assist the education and training of e-Business knowledge with learning systems. According to innovation diffusion theory, we discuss e-Business knowledge (operation of systems, process of systems, and performance evaluation and analysis) and learning systems (e-Learning system, workflow simulation system, and decision simulation system). Finally, results show that e-Learning system costs a few and has direct learning performance. Workflow simulation system and decision simulation system costs a lot, but it has better learning performance. Therefore, this study proposes that innovation diffusion theory is adapted to promote e-Business knowledge with learning systems.