- 期刊
- OpenAccess
The Significance of Mutation in IL-1β Gene and Circulatory Level for Prediction of Trauma Severity and Outcome in Traumatic Cerebral Hemorrhagic Contusion
摘要
Background: Traumatic brain injury (TBI) is a leading cause of death, disability, and resources consumption. Cerebral hemorrhagic contusions are primary brain lesion and often one of the most visible lesions following TBIs. Interleukin-one beta (IL-1β) is pro-inflammatory cytokines it is circulatory level and gene have been implicated in secondary brain injury and worse outcome following TBIs. This study is to determine the significance role of IL-1β gene polymorphism (-511C/T) and circulatory level for prediction trauma severity and outcome in traumatic cerebral hemorrhagic contusion. Methods: The study population includes 90 Sudanese patients with traumatic cerebral hemorrhagic and 90 apparently healthy individuals as control. IL-1β serum concentration was measured using enzyme-linked immunosorbent assay and IL-1β gene was genotyped using restriction fragment length polymorphism-polymerase chain reaction. Results: Significant elevation of IL-1β level was seen among trauma patients compared to control (p-value ＜ 0.001). Although there was no significant association between IL-1β level with trauma severity or death; IL-1β level was higher in severe brain injures compared with moderate and mild one, and the mean concentration of IL-1β was high (18.75 pg/mL) among patient developed poor outcome compared to survivals (15.17 pg/mL). T recessive allele of IL-1β gene was detected in 13.3% of participant. The highest circulatory level of IL-1β (17.8 pg/mL) was observed among patients with TT homozygous alleles. IL-1β gene polymorphism was not associated with trauma severity and death. Conclusions: IL-1β circulatory level was varied according to trauma severity and highly levels were seen among patients developed unfavorable outcome. IL-1β-511C/T gene was not associated with trauma severity and outcome.
延伸閱讀
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