蘭花產業是我國農業發展的旗艦作物,在所有種類的蘭花中,蝴蝶蘭是世界上最重要的盆花作物之一。台灣是世界上最主要的蝴蝶蘭生產國家,2011年台灣的蝴蝶蘭外銷總值已經達到27億台幣。本論文針對蘭菌對於蝴蝶蘭的生長促進、誘導抗病、以及外加植物生長調節物質對於開花品質的增進以及開花時間的調節作了一系列詳細的研究,以期在學術上或實際的產業利用上,有所貢獻。利用蘭菌之接種(Rhizoctonia spp. (R02及R04))可有效促進蘭苗的生長,並且可增強對軟腐病菌(Erwinia chrysanthemi)的抗性。經由抑制性扣減雜合技術(Suppression subtractive hybridization,SSH)的方式,已找到數個可能與抗軟腐病相關的基因。在蝴蝶蘭開花控制時,施用三種不同濃度的三種cytokinins (N-6-benzyladenine(50、100、150 mg·L-1)、kinetin(100、200、300 mg·L-1)及2-isopentenyladenine(50、100、150 mg·L-1))。結果顯示施用N-6-benzyladenine 100及150 mg·L-1對開花的促進效果最佳,可顯著增加花梗數及花朵數。
Orchid industry is the flagship of agricultural crop development in Taiwan. Phalaenopsis, in all type of orchids, is the most important potted flower in the world. Taiwan is the main orchid producer countries in the world. In 2011 the export value of Taiwan's Phalaenopsis orchid has reached NT $ 2.7 billion. This thesis focus on the utility of rhizoctonia to phalanopsis including growth promoting, induce disease resistance, improve the quality of flowering and flowering time regulation. I expect these results can be contributed to the orchid industry and basic research in orchid. Inoculation of Rhizoctonia spp. (R02 and R04) can enhances Phalaenopsis orchid’s growth and resistance to Erwinia chrysanthemi. By suppression subtractive hybridization (SSH) assay, we found several candidate genes which play a role at against Erwinia chrysanthemi. In the other hand, application of three different concentrations of the three cytokinins (N-6-benzyladenine (50、100、150 mg·L-1)、kinetin (100、200、300 mg·L-1) and 2-isopentenyladenine (50、100、150 mg·L-1)) at flowering control. The results showed that application of 100 or 150 mg·L-1 N-6-benzyladenine on flowering for best results, can significantly increase the number and flower stalk number.
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