sOGP10~14 接枝於聚己內酯多元醇-氫氧基磷灰石複合薄膜對於成骨細胞活性之研究

組織再生膜(GTR)可以阻隔生長較快之上皮組織侵入骨質缺損處而影響癒合，目前應用主要之材料以不可吸收性之組織再生膜為主(例如：ePTFE, Gore-Tex®)，但是其生物不可吸收性，需要二次手術將其移除，因此許多人開始研究生物可吸收性之膠原蛋白膜或是高分子聚合膜之材料，膠原蛋白膜雖然有良好之生物相容性，但是其機械強度不足，分解速度與薄膜性質較無法控制，而高分子聚合膜雖然可以改善機械性質，但是生物相容性不佳。本研究的目的主要是自行設計組織再生膜，由具有高度生物相容性的生物陶瓷-氫氧基磷灰石，並在其表面接上骨質生長因子- sOGP10~14，以MG63細胞培養於複合薄膜之上觀察其貼附與存活率之情況，以及檢測鹼性磷酸酶酵素之活性，再以即時定量反轉錄聚合酶鏈式反應（Real-time Quantitative PCR）檢測成骨基因之表現。本研究所自行合成之氫氧基磷灰石結晶已由X光粉末繞射儀檢測確定，在複合薄膜的製備上則由傅利葉紅外線光譜儀(FTIR)檢測證實薄膜表面有聚己內脂多元醇與氫氧基磷灰石之表現，於MG63之培養上，發現複合薄膜在細胞的貼附與存活率測試上比未改質過之薄膜較好，於Real Time-PCR與鹼性磷酸酶活性檢測之實驗中可發現複合薄膜上之氫氧基磷灰石對於鹼性磷酸酶與骨鈣素也有促進之現象。由以上之結果可以得到以氫氧基磷灰石與sOGP10~14進行薄膜表面之改質證實對於細胞之貼附與成骨基因之表現有顯著改善的情況，以及混有氫氧基磷灰石之複合薄膜有應用於牙周骨質再生之潛力。

關鍵字

組織再生膜；聚己內酯多元醇；氫氧基磷灰石； sOGP10~14 ；複合薄膜；表面改質

並列摘要

Guided tissue regeneration (GTR) is a technique which is used for the treatment of bone defects associated with periodontal disease or dental implants. Currently, non-resorbable materials (ex：ePTFE, Gore-Tex®) are used for the fabrication of the membranes. However, a disadvantage of non-resorbable membranes is that they require a second surgical session for the removal of the membrane. Conversely, biodegradable membranes such as collagen and synthetic biodegradable polymers have been studied by many researchers. Collagen membrane has excellent cell affinity and biocompatibility to regenerate tissues; however, its mechanical strength is poor. Biodegradable polymers exhibit improved mechanical properties but cell affinity is not good. Therefore, the aim of this study was to fabricate a composite which is composed of a bioresorbable polymer (Polycaprolactone) and bioactive ceramic (Hydroxyapatite). In addition, osteogenic growth peptide (sOGP10~14) was immobilized on the surface of composite. The osteoblasts activity of MG63 cells including cell attachment, viability, and calcification related gene and enzyme was examined. The characteristic peaks of X-ray diffraction were identified as hydroxyapatite. Successful combination of polycaprolactone and hydroxyapatite was demonstrated by FTIR analysis. Cell adhesion and cell viability onto PCL+HAP+sOGP10~14 composite membrane was elevated after surface modification. The alkaline phosphates enzyme activity and the gene expression level osteocalcin were up regulated by hydroxyapatite. In summary, our results suggest that HAP and sOGP10~14 plays a significant role in stimulating the bone derived cellular activity and hydroxyapatite composite membrane have high potential to be used for hard tissue regeneration.

並列關鍵字

Guided tissue regeneration ； Polycaprolactone ； Hydroxyapatite ； sOGP10~14 ； Composite membrane ； Surface modification

參考文獻

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sOGP10~14 接枝於聚己內酯多元醇-氫氧基磷灰石複合薄膜對於成骨細胞活性之研究

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