薄葉嘉賜木微體繁殖之研究

Study on the micropropagation of Casearia membranacea Hance

周沁澐(Chin-Yun Chou)

指導教授 ： 王亞男

國立臺灣大學/生物資源暨農學院/森林環境暨資源學研究所/碩士(2017年)

https://doi.org/10.6342/NTU201700521

摘要

本研究之目的為建立薄葉嘉賜木組織培養之微體繁殖體系。果實消毒以億力( 1g/L )及鏈黴素( 1g/L )溶液中浸泡1小時，流水處理1小時，再以濃度75%的酒精浸泡並手搖30秒，再浸於5%次NaOCl溶液中，在超音波震盪器10分鐘後移於無菌操作檯，以ddH2O清洗3-4次，在無菌操作台中取出種子或胚進行試管培養，結果顯示無污染率約99.6%，發芽率高於6成，並建立無菌苗的繁殖體系。在器官形成方面:以無菌苗頂芽與帶芽莖段較佳之培殖體，其在含有不同植物生長調節劑的WPM培養基中皆達9成以上的芽體誘導率，其中以添加1mg/L BA及同時添加 1mg/L BA和0.1mg/L NAA的培養基誘導率100.00%最高。另外添加0.5mg/L BA的培養基平均誘導芽體數2.69個最高。不同培殖體間以頂芽誘導芽體率100.00%最高，且以帶多個芽點的莖段產出芽體數2.60最高。此外子葉以及下胚軸亦可以誘導出芽體。發根試驗中，以添加0.1mg/L NAA之WPM培養基誘導率100.00%最高，其餘添加NAA之培養基的發根效果亦佳。此外，未添加植物生長調節劑的與添加活性碳之培養基誘導發根之能力也很好。
由誘導芽體產生之完整小苗經馴化6周後存活率達96%。

關鍵字

薄葉嘉賜木 ； 微體繁殖 ； 無菌苗,馴化 ； WPM

並列摘要

The main objective of this study is to establish the micropropagation system of Casearia membranace Hance.
The fruits are sterilized with 1g/L benomyl and 1g/L streptomycin for 1 hour, and treated with running tap water for 1 hour. The fruits were then shaken in 70% ethanol solution for 30 sec, and soaked in NaOCl with ultrasonic shaking for 10 min afterwards. Next, we washed them with double distilled water for 3-4 times in laminar air flow chamber. Then we took out the seeds or the embryos by aseptic manipulation. The result showed the contamination rate was less than 1%, and the germination rate is more than 60%. This process can successfully establish aseptic plantlets.
In the aspect of organ formation: the aseptic shoot tips and nodals are the best explants for shoot induction. Those cultured in WPM medium with several different PGRs compositions all showed more than 90% induction rate. WPM+1mg/L BA and WPM+1mg/L BA+0.1mg/L NAA medium were the best among them, which showed 100.00% induction rate. In addition, WPM+0.5mg/L BA medium showed the maximum shoots, which was in average 2.69 shoots. Furthermore, cotyledons and hypocotyl segments can also induct shoots. The result of root induction test showed that the WPM+0.1mg/L NAA medium was the best one. In addition, other NAA added mediums were also accessible. Moreover, blank WPM medium and WPM with activated carbon mediums were suitable for root induction for Casearia membranacea at the same time.
Through micropropagation process as above, these complete plantlets achieved 96% survival rate after 6-week acclimatization.

並列關鍵字

Casearia membranaces Hance ； micropropation ； aseptic plantlet ； acclimatization ； WPM

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