前處理對污泥厭氧產氣之影響

Effects of Pretreatment on Anaerobic Digestion of Biological Sludges

詹才武(Tsai-Wuu Jan)

指導教授 ： 李篤中

國立臺灣大學/工學院/化學工程學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.00826

摘要

本論文目的是探討前處理對污泥消化之影響。前處理包含加入微生物菌群、增溫、凍融及調整酸鹼值等。微生物菌群處理是加入梭狀芽孢桿菌群(Clostridium spp.)。實驗中，運用代謝物分析與微生物社群分析(PCR-DGGE)結果來追蹤厭氧消化的代謝現象及菌群之變化。高性能液相層析儀(HPLC)所檢測到的代謝物主要以揮發酸(Volatile Fatty Acids, VFA)。微生物菌群監測目標主要以產酸及產甲烷菌為主，如:Clostridium spp.、Methanobacterium spp.。研究結果發現，污泥加入梭狀芽孢桿菌群培養液後，可大幅加速污泥厭氧消化及增加甲烷產量。然而先經過增溫、凍融及調整酸鹼值處理的污泥再加入梭狀芽孢桿菌培養液的樣品其產氣結果並未明顯優於單純加入梭狀芽孢桿菌群培養液的處理方式。

關鍵字

污泥消化 ； 產甲烷菌 ； 梭狀芽孢桿菌群 ； 揮發酸

並列摘要

The aim of this thesis is intended to investigate the influence of pretreatment on sludge digestion. Microbial inoculums, sterilization, frozen/thawed, acidification, and alkalization are used to pretreat the sludge. The microbial inoculum is clostridium inoculums. In our experiment, we used the HPLC and microbial community analysis (PCR-DGGE) to monitor the digestion pathway and the microbial population change. The major metabolite detected by HPLC are volatile fatty acids (VFA) and lactic acid. The main tracked bacteria in microbial community analysis are acetogens and methanogens, like Clostridium spp.、Methanobacterium spp.. The results show that the pretreatment of Microbial inoculums can substantially increases digestion rate and methane product. However, the sludge treated by adding Clostridium inoculums combined with other pretreatmentis aren't clearly better than only by Clostridium inoculums.

並列關鍵字

sludge digestion ； methanogens ； Clostridium spp. ； volatile fatty acids (VFA)

參考文獻

丁健弘（2004）,活性污泥之厭氧消化產氣,國立台灣大學化學工程所論文,台北。

John G. Holt, editor-in-chief. c1984-c1989. Bergey's manual of systematic bacteriology. Williams & Wilkins. Baltimore.

Amann, R. I., W. Ludwig, and K. H. Schliefer.. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. Mar. :143-169

Bleicher K.; G. Zellner; J. Winter; 1989. Growth of methanogens on cyclopentanol/CO2 and specificity of alcohol dehydrogenase. FEMS Microbiol. Lett., vol. 59, pp. 307–312.

Cord-Ruwisch, Hans-Jürgen Seitz, Ralf Conrad. 1988 Thecapacity of hydrogenotrophic anaerobic–bacteria to compete for traces of hydrogen depends on the redox potential of the terminal electron-acceptor. Arch. Microbiol. 149 (4): 350-357.