- 學位論文
應用多光子螢光及二倍頻顯微術於肝細胞癌診斷
Application of Multiphoton Fluorescence and Second Harmonic Generation Microscopy in Diagnosing Hepatocellular Carcinoma
摘要
肝癌長久以來一直是國人揮之不去夢魘，而國內對於肝癌的研究也是相當重視。但是醫師對於癌症判斷，卻僅止於經驗法則，雖然此法已行之有年，但主觀上判讀仍是存在精確度的疑慮。若有更客觀的方式，去建立一套分析的準則，不僅可以提高癌症級數判讀的準確度，同樣也是輔助醫師的一項利器。 而本實驗室便是利用雙光子螢光與二倍頻顯微術(以下簡稱顯微術)來觀測肝細胞癌(HCC)切片，藉此達到上述之目的。藉著本實驗室顯微術系統，掃描各級數是切片樣本，在擷取其螢光與二倍頻影像，再進一步分析。 將取得之影像對其雙光子螢光或是二倍頻訊號強度，與各種參數分析，試著去找出其中的對應關係與趨勢。而利用這些結果去建立一套較客觀的準則，藉此輔助或是精進癌症程度的判斷。 而目前所得結果顯示，就自發螢光而言，非癌症樣品較癌症樣品高，但仍需較多的樣本數來驗證。並試著藉此結論建立分級標準。
並列摘要
Hepatic disease has always been a major health problem in Taiwan, with hepatocellular carcinoma (HCC) being among the leading causes of death. Since the diagnosis of the degree of liver carcinoma is done according to the doctor’s clinical experience, there is a need for a more objective determination of the extent of this cancer. In this work, we attempt to develop an objective method to help improve the accuracy of HCC classification which will be a useful aid for clinical investigations. In this study, we make use of two-photon fluorescence (TPF) and second harmonic generation (SHG) microscopy in imaging hepatocellular carcinoma (HCC) specimens. By scanning thin sections of different HCC specimens, we attempt to correlate the change in the sample’s autofluorescence with parameters such as tumor size. We hope that this approach can lead to a more accurate classification of HCC. Our preliminary results show that the autoflurescence intensity of non-tumor sample is higher than that of tumor sample. Our approach demonstrates the possibility of using tissue autofluroescence for tumor classification and the potential of this methodology for clinical diagnosis.
參考文獻
延伸閱讀
- Hsieh, C. T. (2010). 利用多光子螢光光譜、顯微術以及螢光生命週期顯微術來診斷肝癌 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.02944
- 吳綠茵、蔡宏名、呂佳興（2014）。應用磁振擴散加權影像檢測肝細胞癌最佳化b值。臺灣醫事放射期刊，2(2)，25-32。https://doi.org/10.6717/JTMRT.201407_2(2).0003
- 孔慶惠、曾旭明、陳嘉美、徐劍耀、蘇誠道、賴明陽、許金川、陳定信、魏達成（1992）。肝細胞癌之電腦斷層掃描－延時顯影技術之探討。中華放射線醫學雜誌，17(4)，369-376。https://www.airitilibrary.com/Article/Detail?DocID=10188940-199212-17-4-369-376-a
- Wu, C. H., Hwang, J. I., Hung, S. W., Chou, Y. H., Lee, T., & Lee, S. K. (2005). 利用雙相螺旋電腦斷層追蹤評估經皮射頻滅除術對肝細胞癌的治療效果. 中華放射線醫學雜誌, 30(2), 65-74. https://www.airitilibrary.com/Article/Detail?DocID=10188940-200505-30-2-65-74-a
- Tseng, J. Y. (2010). Imaging and Quantification of Tissue Glycation by Multiphoton Auto-fluorescence and Second Harmonic Generation Microscopy [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.00730