- 學位論文
建立同步監測尿液中Cotinine與8-OHdG線上固相萃 取質譜分析方法探討餐飲業人員油煙暴露與氧化傷害指標之相關性。
The study of relationship between cooking oil fume exposure and DNA oxidative stress by simultaneous analysis of urinary Cotinine and 8-Hydroxy-2’-deoxyguanosine with On-line Solid Phase Extraction Isotope-dilution and LC-MS/MS.
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並列摘要
Lung cancer has been the first cause of death for a long time in Taiwan. Smoking is considered as the primary carcinogen, but the smoking rate of female is even lower in Taiwan. Epidemiology research shows that cooking oil fume is the significant risk factor of lung cancer. Restaurant workers are study subjects of this research. There were 46 waiters, 59 cooks and 20 vendors recruited to join this study. Urine samples of pre-work and post-work were collected. We use tt-DDE (trans, trans-2,4-decadienal) to represent the exposure of cooking oil fume. Cooks and Vendors used personal air sampling equipment to collect the tt-DDE while they were working. We wanted to develop an LC-MS/MS analysis method for simultaneously quantification of urinary 8-OHdG and cotinine. To monitor the oxidative stress after the exposure of cooking oil fume. The simultaneously analysis method for quantification of urinary 8-OHdG and Cotinine has been established. Quantification range of cotinine is from 0.5ng/mL to 1000 ng/mL. The limit of detection (LOD) is 0.5ng/mL. Accuracy is from -0.22% to 3.76%. Precision is from 0.86% to 7.67%. The quantification range of 8-OHdG is from 0.5ng/mL to 15ng/mL. the limit of detection (LOD) is 0.5ng/mL. Accuracy is from -2.28% to 0.19%. Precision is from 1.42% to 8.24%. R2 values are larger than 0.995. Urinary 8-OHdG concentrations of cooks and vendors are higher than waiters (p-value <0.0001) and urinary 8-OHdG concentrations of post-work are higher than of pre-work. Urinary cotinine concentrations of cooks and vendors are also higher than waiters.( p-value<0.0001)
參考文獻
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延伸閱讀
- 林鈴雅（2004）。暴露於含有多環芳香碳水化合物的工作者其尿液中1-Hydroxypyrene濃度之相關性研究－以廚師與煉焦工人為例〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-0707200816054678
- 吳芸臻（2009）。建立尿液中多環芳香烴代謝物分析方法用以評估一般人多環芳香烴暴露之研究〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-3108200909064500
- 翁昭枝（2010）。建立C1~C10醛類物質的採樣分析方法以評估油煙中醛類物質的濃度分佈之研究〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://doi.org/10.6832/KMU.2010.00096
- Chen, Y. H. (2022). 生鮮及加工肉品中新菸鹼類農藥、對羥基苯甲酸酯類及雙酚類之 ID-UPLC-MS/MS 方法開發、含量測定及暴露評估研究 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU202203700
- 周晏如、蔡朋枝、張振平、何雨芳、謝正悅、陳美如（2013）。Assessing the Acid Values of Cooking Oil, Emission Concentration of Polycyclic Aromatic Hydrocarbons (PAHs) and Their Corresponding Toxicities during the Pre- and Post-Frying。勞工安全衛生研究季刊，21(3)，320-330。https://www.airitilibrary.com/Article/Detail?DocID=10249877-201309-201310110020-201310110020-320-330