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  • 學位論文

栽培環境對虎杖白藜蘆醇含量之影響

Effect of culture environment on resveratrol content of Polygonum cuspidatum.

指導教授 : 張祖亮
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摘要


白藜蘆醇(resveratrol)是一種多酚類(polyphenols)成分,屬於植物防禦素(phytoalexin)的一種。具有抗氧化、抑制腫瘤和心血管疾病預防的作用。虎杖(Polygonum cuspidatum Sieb. et Zucc.)為蓼科多年生灌木狀草本,富含白藜蘆醇等其他多酚二次代謝物,中醫上常用於治療癌症、B型肝炎、高血壓等疾病。本研究採用之虎杖植株,原生於南投縣仁愛鄉梅峰附近山區,由明道大學選育高白藜蘆醇含量之單株經無性繁殖成051111選系;具有高含量二次代謝物,除白藜蘆醇外,尚有白藜蘆醇苷、大黃素等多種具生物活性成份。 這類植物防禦素在植物體內的含量會受到環境影響,本研究逐月探討在埔里、台北、春陽及梅峰露地栽培虎杖,其地上部與地下部的生長情形和白藜蘆醇含量的變化,地上部並以分級方式,研究主枝和側枝各部位間的差異。 二年生虎杖的生長情形以種植於梅峰為最佳,地上部分級採收與地下部鮮乾重分別為55 g/part和401.82 g/plant,乾重13.72 g/part和140.73 g/plant。白藜蘆醇含量以台北最高,地上部與地下部分別為0.04和3.33 mg/g DW。採收時期為八月時地上部與地下部皆有最高採收乾重10.59 g/part和118.83 g/plant,地上部白藜蘆醇含量於五月時達最高0.06 mg/g DW,地下部於八月達最高3.19 mg/g DW。地上部不同部位中的生長量和白藜蘆醇含量以側枝較主枝佳,以側枝的Part5(第4~6節)和Part6(第7節以上)最高。評估白藜蘆醇總產量,地上部於梅峰所產植株於七月時採摘Part6部位最佳(1.98 mg/part),地下部以梅峰所產於八月採收者最佳(561.35 mg/plant)。

並列摘要


Resveratrol(,trans-3,4,5-trihydroxystilebene) is natural plant polyphenols and it is a kind of phytoalexin. It has preventative functions for cardiovascular diseases, anti-oxidative effects and anti-cancer activities. Polygonum cuspidatum Sieb. et Zucc. belongs to Polygonaceae and is classified as perennial shrub rich in resveratrol and other secondary metabolites. In traditional Chinese medicine, it is used for cancer, hepatitis B, hypertension and other diseases. 051111 line was derived from Meifeng and selected by Mingdao University. This line has high levels of secondary metabolites including resveratrol, piceid and emodin, etc. The phytoalexin content in the plants will be effected by environmental factor. Studies to investigate the effects of cultivated location, seasons, harvest parts on resveratrol content and plant growth on Puli、Taipei、Chunyang、Meifong. The growth pattern of aboveground main stem and lateral shoot had been completed for two years old P. cuspidatum. The harvest weight showed an obviously difference between aboveground and underground. Crops two years old and cultivaled at Meifong had the highest growth and harvest parts. Fresh weight of underground parts 55 g/part and 401.82 g/plant, dry weight 13.72 g/part and 140.73 g/plant. The highest resveratrol content was 0.04 and 3.33 mg/g DW of aboveground and underground, respectively. Harvesting in August used 051111 line showing the dry weight of P. cuspidatum aboveground and underground had the highest(10.59 g/part and 118.83 g/plant), the highest resveratrol content of aboveground was in May(0.06 mg/g DW), underground was in August(3.19 mg/g DW). Harvest part showed the weight and resveratrol content of part 5 and 6 better then other. The total resveratrol content showed crops aboveground should choice the part 6 in July at Meifong(1.98 mg/part) and underground harvest in August at Meifong(561.35 mg/plant).

參考文獻


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被引用紀錄


賴鈺文(2015)。不同地區種植的高白藜蘆醇虎杖選系-051111之儲藏穩定性與主要活性成分之關係〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342%2fNTU.2015.01169
鄭安生(2013)。利用白藜蘆醇調控 Nrf2 減緩氧化性損傷以改善糖尿病症狀之研究〔博士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342%2fNTU.2013.03136

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