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  • 學位論文

葉綠素螢光檢測番木瓜罹患木瓜輪點病毒病後之耐受性

Chlorophyll fluorescence measurement distinguishing the tolerance and the susceptibility of papayas(Carica papaya L.) to Papaya ringspot virus

指導教授 : 張龍生
共同指導教授 : 林宗賢(Tzong-Shyan Lin)

摘要


在控制的環境下利用葉綠素螢光儀與可攜帶式光合作用測定儀,分析‘台農二號’番木瓜葉片的基本葉綠素螢光特性並檢測木瓜輪點病毒病對葉綠素螢光的影響,且探討葉綠素螢光與傳統光合作用分析方法的關連性。健康番木瓜葉片7個裂葉的變異螢光量與最大螢光量之比值(the ratio of variable fluorescence to maximum fluorescence, Fv/Fm)無顯著差異。在不同日期調查葉齡對葉綠素螢光的影響,隨時間Fv/Fm逐漸下降,最小螢光量(minimum fluorescence, Fo)逐漸上升。‘台農二號’番木瓜葉片感病5週後,罹病株與健康對照間之各葉綠素螢光參數、淨光合成速率(net photosynthetic rate, Pn)、葉綠素讀值(chlorophyll meter reading, CMR)與葉溫有顯著差異,罹病株Fo上升55%、Fm減少9%,而Fv下降18%,罹病株的變異螢光量與最小螢光量之比值(the ratio of variable fluorescence to minimum fluorescence, Fv/Fo)與Fv/Fm分別只有健康植株的53%與90%,Pn為健康對照的58%,罹病株CMR值顯著低於健康植株且葉溫顯著高於健康植株的葉溫。隨發病程度愈嚴重Fv/Fo、Fv/Fm、Fv、Pn與CMR下降愈多,而Fo會上升,但在發病程度最嚴重的葉片Fo有下降的情況,可能為光合成構造已嚴重損壞之故。迴歸分析葉綠素螢光與傳統光合作用測量所得的數值關連性極高,所以葉綠素螢光可供分析番木瓜葉片光合特性之用。

並列摘要


‘Tainunag No.2’ papaya cultivar is well known and popular in Taiwan, although it is susceptible to Papaya ringspoot virus ( PRSV ). Chlorophyll fluorescence is a tool to characterize photosystematic parameters of papaya plants under biotic stress of infection of the severe mottle with necrosis strain of Papaya ringspoot virus ( PRSV-SMN ) under the growth chamber condition. There were not significantly different of ratio of variable fluorescence to maximum fluorescence ( Fv/Fm ) among leaflets of the papaya leaf and among different leaf orders at phyllotaxy. However, the minimum fluorescence (Fo) in the lower part leaves was significantly higher compared to that of younger leaves while the Fv/Fm decreased. Parameters of chlorophyll fluorescence such as Fo, Fv, Fv/Fm were not significant different difference no matter when you measured during the day or at the night. Five weeks after inoculation of PRSV-SMN, fluorescence parameters, net photosynthetic rates (Pn), chlorophyll meter reading (CMR) and leaf temperature were dramatically changed at the infected leaves compared to that at the control plants. The ratio of variable fluorescence to minimum fluorescence (Fv/Fo), Fv/Fm and Pn significantly decreased 53%, 90% and 58% compared to that at the healthy control plants, respectively. So the disease severity progressed, the change of photosystematic parameters became more serious. Results might indicate that photosynthetic apparatus was serios damage due to virus infection. The viral susceptibility of ‘Thinung No.2’ was apparently able to analyze changes in parameters of fluorescence by chlorophyll fluorescence and gas exchange measurement.

參考文獻


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被引用紀錄


吳建銘(2007)。木瓜輪點病毒(SMN、DF系統)與木瓜畸葉嵌紋病毒在不同番木瓜品系上的交互作用〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342%2fNTU.2007.01261

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