即時檢測汞離子�胰蛋白酶的液晶感測系統

Real-time Liquid Crystal Based Sensor System Detecting for Mercuric ion/Trypsin

林義程(Yi-Cheng Lin)

指導教授 ： 陳志欣

淡江大學/理學院/化學學系碩士班/碩士(2015年)

https://doi.org/10.6846/TKU.2015.01022

摘要

液晶感測系統 (liquid crystal based sensor system) 是一種具有輕巧、便宜、操作簡單等特性的檢測系統，利用液晶在偏光下排列的不同，產生不同的色彩變化，使用者不具有專業知識也可以判斷實驗結果。
　　我們的研究分為兩部分，第一部分為汞離子的檢測，將能夠與汞離子配位的配體添加於液晶，當溶液中含有汞離子時，錯合物於液晶－水溶液界面產生，使液晶方向改變，而液晶的光學訊號會由暗轉為亮。
　　第二部分為胰蛋白酶的檢測，我們希望能藉由簡單的修飾方法，將蛋白質修飾於液晶感測器之金屬網格表面，經由水中胰蛋白酶的水解作用，使蛋白質被水解成片段並破壞液晶排列，使液晶產生光學訊號的轉變，此部分我們使用了三種不同的修飾方法作為液晶檢測的比較。

關鍵字

液晶 ； 胰蛋白酶 ； 汞離子

並列摘要

Liquid crystal sensor is a new type of system that is portable, cheaper and simple operation. Liquid crystal is provided a colorful optical signal according to different orientation of liquid crystals. Therefore, even if the user haven’t professional technique that can use the sensor.
We divide into two parts in our research, first part is the detection of mercury ion. We doped the ligand with the liquid crystals that produce complex in aqueous-liquid crystal interface. The optical signal was change from dark to bright. Second part is the detection of trypsin. We modified the protein on the metal grid. Hydrolytic reactions caused optical signal change. In this part, we used three kind of method to modified the protein.

並列關鍵字

Liquid crystal ； Trypsin ； Mercury ion

參考文獻

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