本研究成功地透過高效率的點擊化學反應合成出以poly (amido amine) (PAMAM) 樹枝狀高分子修飾的兩性C60/PAMAM複合體。此C60/PAMAM複合體具有高度的水溶性,其外圍PAMAM之胺基基團帶有多重正電荷,可與帶有負電荷之核酸結合以當作基因載體。透過乳癌細胞MCF-7與子宮頸癌細胞HeLa的細胞吞噬作用效率之探討,證明此C60/PAMAM複合體具有作為基因載體之能力。而在進一步的研究中,我們將plasmid DNA pEGFP-C1與C60/PAMAM複合體進行基因轉染實驗,發現具有穩定的綠色螢光蛋白GFP的表現。另外,由DPPH自由基清除測試、β-胡蘿蔔素漂白試驗法與Fenton氧化反應降解染料Rhodamine B的結果顯示,此C60/PAMAM複合體可作為有效的自由基清除劑。因此,本研究所合成出來之水溶性C60/PAMAM複合體可做為具有基因載體及抗氧化劑之雙功能新型態生醫材料。
In this research, we have successfully conjugated fullerene (C60) and poly (amido amine) (PAMAM) dendrons via efficient “click reaction” to yield amphiphilic fullerene-PAMAM-dendron bearing multiple positive charges. On the basis of strong binding between cationic C60 adducts and negatively charged nucleic acids, the amphiphilic C60/PAMAM dendron complex could serve as a non-viral vector toward in vitro gene transfection. Cellular uptake study shows that the complexes of C60/PAMAM dendrons surely deliver the pEGFP-C1 into HeLa and MCF-7 cell lines at low nitrogen-to-phosphorous ratio. Therefore, the MCF-7 cell lines could be successfully transfected with the pEGFP-C1, revealing a stable GFP expression. Moreover, DPPH radical scavenging assay, β-carotene bleaching assay, and the assessment of Rh-B decomposition via Fenton reaction indicate that the complexes could exhibit a remarkable ROS scavenging capability. Accordingly, the newly designed materials performed a dual function of antioxidant and gene carrier.
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