Atorvastatin及Pioglitazone預防高脂飲食誘發小鼠高血糖及高血脂之影響

The effect of atorvastatin and pioglitazone in the prevention of high fat diet induced hyperglycemia and hyperlipidemia in mice

黃敬詠(Jing-Yong Huang)

指導教授 ： 劉德中

中山醫學大學/健康管理學院/營養學系碩士班/碩士(2017年)

摘要

本研究以動物模式探討，給予高飽和脂肪飲食小鼠補充低劑量之降血脂藥物Atorvastatin及低劑量之降血糖藥物Pioglitazone，對血糖及血脂升高是否有預防效果。本實驗使用由國家實驗動物中心購入之5週齡大C57BL/6JNarl小鼠，隨機分成5組：1. Normal組，為攝食正常飼料組；2. Control組，為攝食高飽和脂肪酸飼料組；3. A組，為攝食高飽和脂肪酸飼料，並添加低劑量降血脂藥物Atorvastatin組；4. P組，為為攝食高飽和脂肪酸飼料，並添加低劑量降血糖藥物Pioglitazone組；5. A+P組，為攝食高飽和脂肪酸飼料，並混合添加低劑量Atorvastatin及Pioglitazone組。實驗進行8週後犧牲，並分析小鼠心跳血壓、血糖、胰島素、血清尿酸、血液尿素氮、血清及肝臟三酸甘油脂、血清及肝臟總膽固醇、血清肌酸酐、肝臟丙二醛、肝臟丙胺酸轉胺酶、肝臟麩胺酸轉胺酶、肝臟麩胱甘太過氧化酶、肝臟脂肪酸合成酶。分析結果發現，餵食高飽和脂肪酸飲食，並補充Atorvastatin及Pioglitazone，能顯著降低小鼠血壓及胰島素濃度，並使血液尿素氮濃度下降，亦能降低小鼠血中三酸甘油脂及總膽固醇濃度，且補充低劑量之Atorvastatin更能降低小鼠血糖濃度，然而在抗氧化能力方面並沒有提升的效果。但整體而言，補充低劑量Atorvastatin及Pioglitazone對預防高飽和脂肪酸飲食所誘發之血糖及血脂上升是有其功效。

關鍵字

Atorvastatin ； Pioglitazone ； 高血糖 ； 高血脂

並列摘要

The major aim of present study was to examine the protective effect of Atorvastatin and Pioglitazone on hyperglycemia and hyperlipidemia in mice fed the diet rich in saturated fatty acids. C57BL/6JNarl mice were divided into five group: 1. Normal group (fed normal diet); 2. Control group (fed the diet rich in saturated fatty acids); 3. A group (fed the diet rich in saturated fatty acids with low dose Atorvastatin); 4. P group (fed the diet rich in saturated fatty acids with low dose Pioglitazone); 5. A+P group (fed the diet rich in saturated fatty acids with low dose Atorvastatin and Pioglitazone) for eight weeks. The levels of heart rate, blood pressure, glucose, insulin, uric acid, blood urea nitrogen (BUN), serum and liver triglycerides, serum and liver total cholesterol, malondialdehyde (MDA), alanine aminotransferase specific activity, aspartate aminotransferase specific activity, glutathione peroxidase specific activity and fatty acid synthase specific activity were measured. The results showed that the intake of low dose Atorvastatin and low dose Pioglitazone reduced blood pressure, insulin, blood urea nitrogen, serum triglycerides and total cholesterol levels. The intake of low dose Atorvastatin reduced blood glucose level. However, both of Atorvastatin and Pioglitazone cannot increase antioxidant capacity. These data suggest that Atorvastatin and Pioglitazone are potent agents for improving hyperglycemia and hyperlipidemia.

並列關鍵字

Atorvastatin ； Pioglitazone ； Hyperglycemia ； Hyperlipidemia

參考文獻

3. 潘文涵、吳幸娟、葉志嶸、莊紹源、張新儀、葉乃華、謝耀德。台灣人飲食與健康之趨勢：1993-1996與2005-2008兩次營養健康調查之比較。2005-2008台灣營養健康調查。

29. Joel T. Vander Lugt, José Gómez-Márquez, Joel Caldon, Larry L. Louters. Combined effects of troglitazone and muscle contraction. Biochimie, 2001; 83:445-451.

1. USDA Food Composition Databases, 2016.

4. Gimeno-Orna JA, Faure-Nogueras E, Castro-Alonso FJ, Boned-Juliani B. Ability of retinopathy to predict cardiovascular disease in patients with type 2 diabetes mellitus. Am J Cardiol, 2009; 103(10):1364-7.

5. Yorek MA. The role of oxidative stress in diabetic vascular and neural disease. Free Radic Res, 2003; 37(5):471-80.