探討香瓜茄對於老化與長期飲食果糖誘發代謝症候群之研究

Study of Solanum Muricatum regarding the aged and the Long-term Dietary Fructose-induced Metabolic Syndrome

洪筱婷(Hsiao-Ting Hung)

指導教授 ： 張菡馨

中山醫學大學/健康管理學院/營養學研究所/碩士(2009年)

https://doi.org/10.6834/CSMU.2009.00079

摘要

果糖消耗量增加所伴隨的後續效應日益嚴重，由於代謝症候群的高危險性和致死率是目前己開發國家主要的健康危機，然而著眼於過去文獻有關果糖誘發代謝症候群中相關危險因子之研究，發現大多是著重於短時間介入高劑量果糖且只針對單一症狀探討。因此，本研究將分為三大部分(1) 觀察小鼠不同週齡之生理代謝變化；(2) 20%果糖水對於動態生理變化之衝擊效應；(3) 以20%果糖飲水建立代謝症候群之動物模式，共介入21週，同時整合性探討生理代謝、氧化壓力變化、致病機轉及探討香瓜茄是否能有效降低代謝症候群之危險因子的評估分析，並觀察組織形態和功能上的病理改變，有利於縱深研究其對代謝器官之衝擊效應。結果顯示伴隨小鼠週齡增加則使其體重增加、血脂上升及抗氧化酵素活性被削弱之情形(p<0.01)。飲食中添加果糖導致生理代謝方面出現異常，同時在體重、血壓、血糖、總膽固醇皆有顯著上升(p<0.01)，且在腎功能方面，肌酸酐值與尿酸值亦顯著增加(p<0.01)；而氧化壓力方面則造成肝及腎臟其脂質過氧化程度顯著增加(p<0.01)，以及穀胱甘肽(glutathione；GSH)與觸酶(catalase；CAT) 的抗氧化酵素活性顯著下降(p<0.01)；在組織病理學方面顯示肝、腎組織有明顯脂肪堆積情形，且鮑氏囊有顯著紅血球浸潤情形。而經過香瓜茄治療後，其體重有顯著下降(p<0.01)，同時血中尿素氮和肌酸酐的含量也降低(p<0.01)，除此之外，也提升體內觸酶的活性(p<0.01)，但在血脂、肝功能及脂質過氧化方面卻未能達到改善，甚至有出現惡化的趨勢發生。根據本研究發現老化過程中，可能會引起一連串的代謝器官功能衰退效應產生，更進一步了解的是老化將會受到相關因子刺激而加速其進展之過程。另外，飲食中攝入果糖可能會導致肥胖、血壓上升及血脂代謝異常的現象發生，同時合併組織脂質過氧化程度增加，伴隨著體內抗氧化酵素活性出現削弱的趨勢，在肝臟、腎臟皆有觀察到脂肪堆積的情形。因此，我們認為飲食中攝入果糖所帶來的衝擊效應將更值得被關注，一方面是希望未來能訂定飲食中果糖的安全使用劑量；另一方面則是建立改善代謝症候群之有效健康食品，將提倡響應未來養生保健的趨勢發展。

關鍵字

果糖 ； 代謝症候群 ； 肥胖 ； 抗氧化 ； 香瓜茄

並列摘要

Increasing consumption of fructose is getting more serious nowadays. Due to the high risk of metabolic syndrome (MS) and lethal rate is the major crisis in developed countries at present. Focus on previous studies associated with the risk factors for MS induced by fructose purposed to use high dose in short-term and to discuss only single symptom, moreover, they didn’t pay attention on overall condition. Therefore, this study will divide three parts. (1) To observe physiological metabolic changing in different stages compared with the weeks for mice, (2) to observe the detrimental effect of 20% fructose dietary drinking water on physiological metabolic changing in different stages compared with the weeks for mice, (3) Set up animal model of MS by 20% dietary drinking fructose water, intervened for completely 21 weeks, at the same time, we discuss physiological metabolism, variation of oxidative stress, analysis S. muricatum whether effectively reduce risk factors or not, furthermore, observe the morphological, histological and functional changing in pathology to reveal underlying mechanism, which benefited the deeply research in the metabolic organs. The results showed that weight, blood lipid increased and anti-oxidative enzyme activity decreased, mice age increasing (p<0.01). Dietary fructose consumption increasing lead to abnormally physiological metabolism, all of the weight, blood pressure, fasting blood sugar and total cholesterol have notable rising (p<0.01). However, damage to liver and kidneys of lipid peroxidation level significant increased (p<0.01) and the anti-oxidative activity of glutathione (GSH) and catalase (CAT) was significantly decreased (p<0.01). Fat obviously accumulated in liver and kidneys and the blood red cells infiltrated in Bowman’s capsule in histopathology. After treatment by S. muricatum, body weight obviously decreased (p<0.01), besides, the blood urea nitrogen (BUN) and creatinine (CRE) in blood significantly decreased (p<0.01), in addition to, the activity of CAT promoted (p<0.01). However, blood lipid, liver function and lipid peroxidation level do not significant improve. According to this study, we found that fructose could lead to obesity, unusual of blood lipid metabolism and raise blood pressure. Increasing lipid peroxidation level in tissues accompanied with the weaken activity of anti-oxidation activity, lipid accumulating in liver and kidneys is obviously. As a result, we think that detrimental effect of fructose intake should be noticed. Setting up safe dose in dietary fructose is the most important thing in the future. In another part, establishing the effective healthy food to improve MS and promoting to keep in good health and care in the future.

並列關鍵字

fructose ； metabolic syndrome ； obesity ； anti-oxidative ； S. muricatum

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