染布青萃出物對於脂多醣體誘發小鼠RAW264.7巨噬細胞發炎反應之探討

The influence of Indigofera suffruticosa Extracts on LPS-induced inflammatory reaction in mouse RAW264.7 macrophage cells.

朱美慈(Mei-Tzu Chu)

指導教授 ： 劉德中

中山醫學大學/健康管理學院/營養學研究所/碩士(2009年)

https://doi.org/10.6834/CSMU.2009.00102

摘要

染布青，學名： Indigofera suffruticosa Mill，莖葉可提取藍色染料，為民間熟知的靛藍染料來源，故名染布青。
染布青之水溶液萃出物與甲醇萃出物，在每公斤體重50毫克與每公斤體重2.4公克單一劑量毒性試驗下，顯示染布青萃出物對於大鼠無顯著性的毒性 (p >0.05)。
在脂多醣（lipopolysaccharides, LPS）誘發小鼠RAW264.7巨噬細胞發炎反應之模式中，染布青水溶液萃出物與甲醇萃出物對於細胞的存活率無顯著差異 (p >0.05)。染布青水溶液萃出物與甲醇萃出物均有抑制細胞一氧化氮 (NO)生成以及細胞內誘導型一氧化氮合成酶 (iNOS)蛋白質表現之能力 (p <0.05)。但是對於細胞內COX-2蛋白質之表現，染布青水溶液萃出物與甲醇萃出物無抑制效果 (p >0.05)。
由以上的實驗結果可知，染布青萃出物透過抑制iNOS之表現以及降低一氧化氮濃度，達成抑制LPS誘發小鼠RAW264.7細胞發炎效應。

關鍵字

染布青 ； 毒性試驗 ； 發炎反應 ； 一氧化氮 ； 巨噬細胞RAW264.7

並列摘要

Indigofera suffruticosa Mill is the source of dye indigo and a perennial Fabaceae shrub plant. The blue indigo is obtained from the extract of leaves.
In this study, both aqueous extracts and methanol extracts of the whole Indigofera suffruticosa Mill have no toxicity effect on Sprague Dawley (SD). In the model system of lipopolysaccharide(LPS)-induced inflammatory reactions in mouse RAW264.7 macrophage cells, both aqueous extracts and methanol extracts of Indigofera suffruticosa Mill can reduce the production of nitric oxide. In addition, they can also inhibit the expression of iNOS in mouse RAW264.7 macrophage cells. In conclusion, the aqueous extracts and methanol extracts of Indigofera suffruticosa Mill can suppress the immune response of LPS-induced inflammatory reactions in mouse RAW264.7 macrophage cells through the inhibition of iNOS express and suppress the production of nitric oxide.

並列關鍵字

Indigofera suffruticosa Mill ； toxicity test ； inflammation ； nitric oxide ； mouse RAW264.7 macrophage cell

參考文獻

Akaike, T., Inoue, K., Okamoto, T., Nishino, H., Otagiri, M., Fujii, S., and Maeda, H. (1997). Nanomolar quantification and identification of various nitrosothiols by high performance liquid chromatography coupled with flow reactors of metals and Griess reagent. Journal of Biochemistry 122, 459-466.

Barros, G.M.C.C., and Teixeira, S.d.P. (2008). Pharmacobotanical studies of wild indigo species (Indigofera suffruticosa and Indigofera truxillensis, Leguminosae,) with pharmacological properties. Revista Brasileira de Farmacognosia 18, 7.

Chen, Y.C., Shen, S.C., Lee, W.R., Hou, W.C., Yang, L.L., and Lee, T.J. (2001). Inhibition of nitric oxide synthase inhibitors and lipopolysaccharide induced inducible NOS and cyclooxygenase-2 gene expressions by rutin, quercetin, and quercetin pentaacetate in RAW 264.7 macrophages. J Cell Biochem 82, 537-548.

Cohen, J. (2002). The immunopathogenesis of sepsis. Nature 420, 885-891.

Cook, N.C. (1996). Samman, S. J Nutr Biochem 7.