探討龍葵水萃取物對紐西蘭大白兔肝病變之抑制作用

Evaluating the Inhibitory effect of Aqueous Extracts of Solanum Nigrum on High Fat Diet Induced Liver Damage in New-Zealand Rabbit

葉大銘(Da-Min Yeh)

指導教授 ： 李彗禎

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2007年)

https://doi.org/10.6834/CSMU.2007.00129

摘要

脂肪肝一般在臨床上是沒有症狀的，但是有一些危險因子如肥胖者、糖尿病患者或高血脂症，則很容易由單純脂肪變性逐步發展成肝發炎、肝臟纖維化、到肝硬化的地步。它們的病理機轉並不完全了解，但是依據一些研究文獻的報導，可以推測與脂肪酸代謝異常、胰島素阻抗性及氧化壓力所引發的脂質過氧化反應與異常細胞因子的產生有關聯性。
根據東西方的醫學文獻報導指出，龍葵 (學名Solanum nigrum )具有抗氧化、降血脂與保護肝臟等醫學功能。因此本實驗以高脂肪飲食(high fat diet;HFD)餵食紐西蘭大白兔，並於飼料中添加0.25%、0.5%、1% 龍葵水萃取物 (Solanum nigrum water-extractions, SNWE)觀察肝臟的變化。同時在第0週與第8週時給予肝臟超音波掃描，由結果發現SNWE可減低肝臟之回音。經十週後犧牲，結果發現高脂肪會增加紐西蘭大白兔血清AST、ALT、TG、Cholersterol、及LDL-C，減低 HDL-C與 ALB，結果發現添加龍葵水萃取物降低AST、ALT、TG並增加HDL-C及ALB。同時也觀察抗氧化指標Catalase、GSH及測定TBARS來觀察脂質過氧化的情形。
比較HFD處理之大白兔的catalase 、GSH 與餵不同濃度的SNWE的結果顯示，在餵食0.25%、0.5及1%SNWE明顯提升catalase 7.14%、107.14%、117.85%與GSH 16.36%、48.17%及58.18%的濃度。以TBARs測定肝細胞脂質過氧化程度的分析，HFD可以引起肝臟組織lipid peroxidation(LPO)的增加(P<0.05)，而在餵食0.25%、0.5及1%較HFD組分別降低了9.08%，7.26%及32.42%，因此SNWE處理可降低HFD所引起的氧化性損傷並減低肝臟中所發生的脂質過氧化反應，降低肝臟損傷程度。
綜合生化值測定、脂質過氧化程度的分析與抗氧化酵素測定的結果，認為SNWE可以降低HFD引起之肝臟受損的程度而達到保肝的作用。對於未來保健食品的研究提供了更明確的目標。

關鍵字

龍葵水萃取物 ； 紐西蘭大白兔 ； 肝病變

並列摘要

In general, the fatty liver is no symptoms in clinic, but there are some risk factors impending the fatty liver to develop the inflammatory, fibrosis and cirrhosis progressively. The mechanism is not clear, but according several reports from the literature, the mechanisms is correlation with the abnormal fatty acid metabolism, associated with insulin resistance and oxidative stress induced lipid peroxidation and abnormal production of cytokine.
The solanum nigrum water extracts have many medical functions including the antioxidant, lowering lipid and protecting the liver, etc. In this study, high fat diet fed with New-Zealand rabbit and mixed with 0.25%、0.5% and 1% SNWE in fodder to observe the liver changing, meanwhile, the rabbit received two times of abdominal sonographic scanning at initial week and eighth week, respectively. Ten weeks later, animals were sacrificed; and we found the biochemistry value including AST, ALT, TG, cholesterol and LDL-C are increased in value, HDL-C and Alb are decreased in value. When given the SNWE, the AST, ALT and TG is decreased but HDL-C&Alb are elevated. As the same times, the marker of antioxidant such as catalase and GSH are measured, and the lipid peroxidation is detected by the method of TBARs.
Compared the catalase & GSH in different group－the HFD group and different percentage of SNWE in HFD including 0.25%、0.5% and 1% SNWE are marked elevation of activity of catalase 7.14%、107.14% and 117.85%, respectively; and concentration of GSH 16.36%、48.17% and 58.18%, respectively. Using the TBARs for evaluation of lipid peroxidation in liver, the HFD can increase the liver peroxidation (P<0.05). Feeding with 0.25%、0.5% and 1% SNWE reveal lower concentration in 9.08%、7.26% and 32.42% respectively. Treated with SNWE can lower the oxidative stress induced liver damage, and decrease of the damage effect of the lipid peroxidation within liver to protect the liver.
According the biochemistry test, measrued lipid peroxidation and checkup the concentration of antioxidants, the SNWE can protect the liver from the damage caused by HFD; and the results are also offering a new research aim for health food in the future.

並列關鍵字

Aqueous Extracts of Solanum Nigrum ； New-Zealand Rabbit ； Liver Damage

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