探討丹參酚酸B鎂鹽於葡萄糖與氧氣剝奪環境對神經細胞之作用

The study of Magnesium Lithospermate B from Savia Miltiorrhiza at Oxygen and Glucose Deprivation on Neuron Cells

林韋誌(Wei-Jhih Lin)

指導教授 ： 麥富德

臺北醫學大學/醫學院/醫學科學研究所/碩士(2012年)

https://doi.org/10.6831/TMU.2012.00065

摘要

根據行政院衛生署2010年統計，腦血管疾病位居國人十大死因第三名，其中腦中風是腦血管疾病死亡主因。現代人飲食、生活習慣與日常作息的改變，伴隨著年紀增長、遺傳與慢性病，使得腦血管病變與腦血管堵塞的機會變大。腦中風發生時經常是突發性的，短時間內若不能及時治療，腦神經則因缺乏氧氣與養分而死亡，導致身體機能永久的失能，不僅造成自身的不便，也對家庭造成極大的負擔。目前腦中風的治療方式一般都是給予抗血栓藥劑，排除堵塞區域，但此方式可能已經造成部分的腦神經細胞死亡卻不察，以致復發率也極高。
參考中草藥典籍中，發現丹參過去被用於活血去瘀、安神、清熱與涼血消廱等功效。近期有文獻指出其含量最多的水溶性物質丹參酚酸B鎂鹽，對於腦缺血具有降低細胞死亡的效用。本篇論文研究設計一套環境模擬中風情況，將神經細胞分別培養在正常環境與模擬中風環境下，並給予不同濃度丹參酚酸B鎂鹽，觀察對於生長抑制、細胞內含氧自由基、細胞核與鈣離子的差異變化，並且首次嘗試使用飛行式二次離子質譜儀來觀察分析細胞內鈣離子的變化。實驗結果發現經低濃度丹參酚酸B鎂鹽處理後，無論是正常環境還是糖氧剝奪環境下，對於神經細胞的生長抑制沒有明顯差異。隨著處理丹參酚酸B鎂鹽的濃度逐漸提高，細胞內的含氧自由基，無論是在正常環境還是糖氧剝奪環境下，都有降低的趨勢；另外隨著丹參酚酸B鎂鹽的濃度逐漸提高，在正常環境下，濃縮的細胞核數量增加，但在糖氧剝奪環境下，細胞核濃縮的現象有減少的現象。在正常環境還是糖氧剝奪環境下，對於細胞中的鈣離子，分別使用鈣離子螢光染劑與飛行式二次離子質譜儀觀察分析，結果在糖氧剝奪環境下給予丹參酚酸B鎂鹽具有降低細胞內鈣離子濃度。由此可知，丹參酚酸B鎂鹽可以降低在糖氧剝奪環境下，神經細胞內含氧自由基與鈣離子濃度，使得神經細胞的細胞核內染色質濃縮減少，降低神經細胞走向死亡。

關鍵字

丹參酚酸B鎂鹽 ； 糖氧剝奪 ； 飛行式二次離子質譜儀

並列摘要

According to 2010 statistics the Department of Health, cerebrovascular disease, the third leading cause of death among the people, of which stroke is a cerebrovascular disease causes of death. diet, lifestyle and daily routines changes, along with the older growth, genetic and chronic diseases, cerebrovascular disease and cerebral vascular blockage of the larger opportunity. Stroke occurs is often sudden, If not treated in a short time, due to lack of oxygen and nutrients leading to brain death, body out of control, not only cause inconvenience to themselves, but also cause great burden on the family. Current stroke treatment are usually given anti-thrombotic agents, exclude the plug region, this approach may have caused some cell death is not observed, so that the recurrence rate is high.
In reference to the Chinese herbal medicine books, Danshen in the past been used for the efficacy of promote blood circulation, remove blood stasis, relieve vexation, cool the blood to relieve carbuncles and clear away heat. Recent studies put emphasis on water-soluble components, Magnesium lithospermate B, that can reduce the effectiveness of cell death for cerebral ischemia. In this thesis research to design an environmental simulation stroke. Nerve cells were cultured in a normal and oxygen and glucose deprivation environment, and treated with different concentrations of Magnesium lithospermate B, Observed intracellular growth inhibition, free radicals and calcium, and try to use the Time-of-flight Secondary Ion Mass Spectrometry. The results showed that no significant differences in the nerve cell growth inhibition by low concentrations of Magnesium lithospermate B treatment, whether it is the environment of the normal or oxygen and glucose deprivation. With a gradual increase in concentration of Magnesium lithospermate B, the oxygen free radicals in cells, either in the environment of the normal or oxygen and glucose deprivation has decreased. In addition, as the concentration of Magnesium lithospermate B gradually improved, under normal circumstances, the nuclei increase in the number of condensed, oxygen and glucose deprivation of the environment, nuclear condensation phenomenon is declining. For intracellular calcium, Oxygen and glucose deprivation treat Magnesium lithospermate B to reduce the intracellular calcium concentration. Therefore, Magnesium lithospermate B can reduce the nerve cells to die in the environment of oxygen-glucose deprivation.

並列關鍵字

Magnesium Lithospermate B ； Oxygen and Glucose Deprivation ； Time-of-flight Secondary Ion Mass Spectrometry ； TOF-SIMS

參考文獻

行政院衛生署。2010。統計資料台灣地區主要死亡原因。台灣台北。

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