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  • 學位論文

IL-12及IL-16於牙周病的探討

IL-12 & IL-16 in Periodontal Disease

指導教授 : 蔡吉政
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摘要


許多免疫系統(immune system)的活化,在牙周炎的引發及病理過程中扮演著重要的角色,在牙周炎的病灶位置,會有大量具活性的B細胞、漿細胞(plasma cell)及具活性的T細胞。由老鼠體內的樹狀細胞所產生的IL-12,決定了Th1/Th2的平衡狀態,高量的IL-12會促使免疫反應傾向於Th1 type,IL-12除了可以促進Th1細胞的免疫反應,也會抑制Th2細胞的免疫反應。IL-16能引發CD4﹢細胞上IL-2 receptor 的高親和性,而在延遲性過敏反應(DTH)中,IL-16對於T h1細胞及CD4﹢T細胞有趨化性。但,IL-12及IL-16與牙周炎之間的關係並不明確。本研究從19位牙周病患者與6位健康者收集130個牙齦溝液標本,並紀錄其臨床牙周指數、抽菸及飲酒的習慣,以酵素連結免疫吸附分析法測定牙齦溝液標本中之IL-12及IL-16的含量。結果顯示: (1)在IL-12總量上,牙周病組(慢性牙周炎組與牙齦炎組)明顯高於健康組,牙周炎部位高於牙齦炎部位及健康組。IL-12總量與PLI、GI、PD與CAL有正相關。(2)IL-16總量在牙周病組明顯高於健康組,而牙周炎部位則高於牙齦炎部位。IL-16總量則與PD與CAL有正相關。(3)有飲酒者的牙周炎部位,其IL-16濃度與總量明顯高於沒有飲酒者。有吸菸者的牙周炎部位,其IL-16濃度與總量明顯高於沒有吸菸者。(4)有吸菸者其PD與CAL明顯大於沒有吸菸者。因此,吸菸及飲酒會影響IL-16進而促使牙周炎破壞增加。未來的可進一步探討吸菸、飲酒、牙周治療對 IL-12與IL-16的影響,包括如這兩種習慣的年攝取量及牙周治療模式等。

關鍵字

IL-12 IL-16 牙齦溝液 吸菸 飲酒

並列摘要


Immune system plays an important role in the pathological process of periodontitis. A great number of active B cells, T cells and plasma cells exist in the lesion of periodontitis. In mice, IL-12 from dendritic cells determines the balance between Th1 and Th2; high IL-12 will contribute the immune reaction to Th1 type. In addition, IL-12 inhibits the Th2 type immune response. IL-16 can cause the high affinity of IL-2 receptors on CD4﹢cells and is chemotaxis to Th1 cells and CD4﹢T cells. However, the information on IL-12, IL-16 in periodontitis is not clear. In this study, 130 GCF samples were collected from 19 periodontal disease patients and 6 healthy ones. The clinical periodontal indices、the habits of cigarette smoking and alcohol drinking were recorded. The levels of IL-12 and IL-16 in the GCF were determined by ELISA. The results revealed: (1) The total amount of IL-12 is significantly higher in periodontal diseased subjects (chronic periodontitis and gingivitis patients) than in healthy subjects, is significant higher in chronic periodontitis sites than gingivitis or healthy sites, and is correlated with PLI、GI、PD and CAL. (2) The total amount of IL-16 is significant higher in periodontal diseased subjects than in healthy subjects, is significant higher in chronic periodontitis sites than in gingivitis sites, and is correlated with PD and CAL.(3) IL-16 concentration and the total amount of chronic periodontitis are higher in cigarette smokers or alcohol drinkers than in no-smokers or no-alcohol drinkers respectively. (4) The PD and CAL were greater in smoker than non-smoker. Smoking and alcohol drinking would affect IL-16 levels, therefore could have an effect on the destruction of periodontium in periodontitis. The future study could include the effect of smoking、drinking and periodontal treatment on IL-12、IL-16 in periodontitis including the annual consumption of cigarette and alcohol , the phase I periodontal treatment.

並列關鍵字

IL-12 IL-16 GCF smoking alcohol drinking

參考文獻


1. Seymour GJ. Possible mechanisms involved in the immunoregulation of chronic inflammatiory periodontal disease. J Dent Res 1987;66:2-9
2. Seymour GJ. Importance of the host response in the periodontium. J Clin Periodontol 1991;18:421-426
3. Cole KC, Seymour GJ, Powell RN. The autologous mixed lymphocyte reactions(AMLR) using periodontal lymphocytes. J Dent Res 1986;65:473, abstract no. 16.
4. Taubman MA. Stoufi ED, Ebersole JL, Smith DJ. Phenotypic studies of cells from periodontal disease rissue. J Periodont Res 1984;19:587-590
5. Mosmann TR, Sad S. The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 1996;17:138-146

被引用紀錄


吳章瑜(2007)。三黃瀉心湯抑制細胞發炎機制的探討〔碩士論文,中原大學〕。華藝線上圖書館。https://doi.org/10.6840%2fcycu200700859

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