四鈣磷酸鹽骨水泥添加富含血小板血漿之體外性質研究

The characteristic properties of tetracalcium phosphate bone cement with platelet-rich plasma powders as identified through in vitro testing

王縈慧(Ying-Hui Wang)

指導教授 ： 陳文正

共同指導教授 ： 洪純正(Chun-Cheng Hung)

高雄醫學大學/口腔醫學院/口腔衛生科學研究所碩士班/碩士(2009年)

摘要

磷酸鈣陶瓷擁有高生物相容性和生物活性且在臨床上已使用了很多年。生長因子已被證實能促進組織修復。本研究的目的主要是評估四鈣磷酸鹽為主的骨水泥（tetracalcium phosphate-based bone cement (CPC)）添加了富含血小板的血漿（platelet-rich plasma (PRP)）後，在體外（in vitro）和體內（in vivo）實驗的效果。本實驗所使用的PRP粉末是一種商業化的產品（Regenplex™, Hemogen Bio-Tech Co., Taiwan）。我們利用CPC添加或不添加10 wt% PRP浸在不同時間點來研究材料相關的特性。所有樣本會進行抗壓強度、XRD、FTIR和顯微結構分析。CPC(對照組)、CPC添加PRP(實驗組)和取自腸骨(ilium)的自體移植骨(控制組)被植入兔子後肢大腿骨遠端內踝，在手術後的3、6、9週，將兔子犧牲後取得組織切片樣本。CPC添加PRP的組別在抗壓強度、楊氏係數和氫氧基磷灰石的相轉換率都呈現降低之趨勢。根據對兩種CPC材料測試的組織切片資料來說，單純只有CPC植入在術後6和9週其骨頭再生的效果都比CPC添加PRP植入來的不好。而自體移植骨的吸收率太高以至骨生長速度無法配合，所以觀察到移植區域充滿脂肪組織和很少的骨小樑。根據本研究的動物體內實驗結果得知，CPC添加PRP可以縮短骨再生所需的時間。

關鍵字

四鈣磷酸鹽骨水泥 ； 富含血小板血漿

並列摘要

The aim of this study was to evaluate the effects of a platelet-rich plasma (PRP) additive on tetracalcium phosphate-based bone cement (CPC) performance in vitro and in vivo. The PRP powder used was a commercialized product (Regenplex™, Hemogen Bio-Tech Co., Taiwan). We used CPC with and without the 10 wt% PRP to characterize relevant material properties. The samples were examined at different immersion times using strength, XRD, FTIR, and morphologic analyses. Materials
made of PRP/CPC, CPC, and an autograft taken from the ilium were implanted in the medial condyle of a rabbit femur, and the animals were sacrificed to obtain histological samples at 3, 6, and 9 weeks post-operation. Adding the PRP powders into the CPC samples caused a significant reduction (p > 0.05) in the strength, modulus, and the phase transformation ratio of apatite. Histological data from testing
with both CPCs suggested less bone regenerative efficiency in the case of CPC alone at 6 and 9 weeks post-implantation than in the case of the PRP/CPC specimens. The high resorption rate of the biomaterials resulted in adipose tissue filling the implant sites, and very little trabecular bone was observed. The addition of PRP to CPC to form the pastes that were subsequently used for osteoregeneration led to promising data from in vivo testing.

並列關鍵字

tetracalcium phosphate bone cement ； platelet-rich plasma

參考文獻

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