脯氨酸導引蛋白質激酶FA在乳癌與肺癌上皮-間質轉化調節的腫瘤-間質協調進化中扮演的角色

Role of PDPK FA in EMT-mediated tumor-stroma co-evolution of breast and lung cancers

阿依肯(Kali Aikyn)

指導教授 ： 楊孝德

國立清華大學/生命科學院/分子與細胞生物研究所/碩士(2010年)

摘要

腫瘤和基質間的協調串擾與病理相關的上皮-間質轉化對于腫瘤細胞侵襲和轉移是至關重要的。這兩種機制是在腫瘤進展中共存且不可分割的機制。脯氨酸導引蛋白質激酶FA已被闡明爲多底物-多功能的與人類癌症相關的脯氨酸導引蛋白質激酶。在此研究中我們以免疫組織化學爲研究方法觀察了乳癌和肺癌的樣本，並且發現了脯氨酸導引蛋白質激酶 FA 相關的重要細胞族群和它們的腫瘤發展中的上皮-間質轉化-協調的動態。我們又找到了和脯氨酸導引蛋白質激酶 FA密切相關的促使腫瘤的因子，譬如骨橋蛋白和白細胞介素-6。我們的實驗結果表明脯氨酸導引蛋白質激酶 FA是牽涉到乳癌和肺癌的上皮-間質轉化-協調的間質轉化調節進化中的重要信號分子。

關鍵字

脯氨酸導引蛋白質激酶

並列摘要

Coordinated crosstalk between tumor and stroma and pathologically relevant epithelial-mesenchymal transition are vital for tumor cell invasion and metastasis. These two mechanisms are unlikely individual events of tumor progression, instead they are ‘inseparable body’ of tumor progression. Proline-directed protein kinase FA (PDPK FA), which has been characterized as a multisubstrate-multifunctional PDPK possibly associated with human cancers. In this study we investigated human breast and lung clinical tumor samples by means of immunohistochemistry method, and identified crucial nPDPK FA associated cell populations and their EMT-mediated dynamics during tumor progression. We also identified most frequently PDPK FA associated tumor-promoting factors such as osteopontin and interleukin-6. Our data indicates that PDPK FA is a key signaling molecule involved in EMT-mediated tumor-stroma co-evolution of breast and lung cancers.

並列關鍵字

PDPK FA ； tumor microenvironment ； EMT

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