以脈衝式電場掃流超過濾分離牛血清蛋白溶液

Utilizing pulsed electric fields in crossflow ultrafiltration of BSA solution

陳信宇(Hsin-Yu Chen)

指導教授 ： 莊清榮

中原大學/工學院/化學工程研究所/碩士(2002年)

https://doi.org/10.6840/cycu200200219

摘要

掃流過濾已廣泛應用於生化產品之濃縮分離，在掃流過濾時若伴隨電場，結合剪應、電泳及電滲透作用等對濾速的提升應有助益，但是電能消耗是其能否為業界接受所必需考慮的問題。本研究以BSA溶液進行電場脈衝超過濾實驗，探討電場脈衝頻率及電場強度等對濾速、穿透率及能源消耗等的影響。
在定電場強度掃流過濾實驗中，各薄膜之穩定濾速皆隨電場強度增加呈線性提高，而100kDa膜所得之ke值與所量測之BSA電泳移動度相近，而因濃度極化及膜面電場分佈不均等因素，使其他薄膜之ke值與BSA電泳移動度有所差距。在10k~300kDa膜之實驗結果中，僅100kDa膜所得之穿透率與電場強度有較明顯之關係，其隨電場強度增加而降低，推測為其MWCO僅稍大於 BSA分子量，使膜孔周圍之局部高電場強度抑制BSA穿入膜孔所致。
脈衝電場施加方式之結果顯示，除了300kDa膜外，濾液量均隨電場頻率增加而有些微之增加。較長操作時間結果指出，定電場者濾速仍有衰減之現象，脈衝電場操作者，電場施加與中斷時之濾速皆維持於一穩定範圍，且收集之濾液量亦僅稍低於定電場者。
因300kDa及10kDa膜之MWCO與BSA分子量差距甚大，所以脈衝電場頻率對其穿透率影響不明顯，只有100kDa膜之穿透率隨脈衝頻率增加而減少。
由於脈衝電場操作之濾液量僅稍低於定電場之結果，但其卻可明顯降低能源之損耗，所以脈衝電場於薄膜過濾中有其發展潛力。

關鍵字

穿透率 ； 牛血清蛋白 ； 電場過濾 ； 超過濾 ； 脈衝電場

並列摘要

The combination of shearing action and electric field in crossflow filtration has been recognized as an effective means of reducing both concentration polarization and membrane deposition. However, the process has not yet received wide acceptance. One of the reasons for that is the excessive operation cost due to a high energy requirement. In order to minimize the power consumption, attention has been directed to the use of pulsed electric field. There have been brief reports that the use of pulsed electric field can lead to substantial improvements in filtration rates of microfiltration, but so far, the effect of an pulsed field on the performance of ultrafiltration has been studied in few cases.
In order to investigate the characteristics of pulsed electro-ultrafiltration for separating protein solution, experiments with BSA solution by a flat-channel filter were conducted to show the effect of electric field strength and pulsed frequency on the filtration rate, protein retentivity and power consumption under various MWCO PES membranes.
When the filter was imposed with constant electric field strengths, its filtration rate increased almost linearly with the strength, but the slopes of the linear plotting have some deviations from that of BSA mobility determined using electrophoretic measurement. For the cases with 10 and 300 kDa membranes, the electric field strength only gave a little effect on the transmission, but there was an obvious decrease of transmission with the electric field strength when a 100 kDa membrane was used.
The phenomenon that an instantaneous change of filtration rate was associated with the imposed on or off of the pulsed electric field indicated clearly that the electroosmosis plays the major role in enhancing the filtration rate. Due to the amount of filtrate received from pulsed operations was only slightly small as compared with that using constant electric field strength, the former has an obviously high electric energy efficiency based on per unit mass of filtrate. The another advantage of this method is that the thermal effect which is a drawback for heat sensitive materials can be avoid. In addition, it was also found that the gradual attenuation of filtration rate in the duration with constant electric field strength was almost not observed in the pulsed processes except with the large MWCO such as 300 kDa membrane.

並列關鍵字

Transmission ； Pulsed electric field ； Ultrafiltration ； BSA ； Electro-filtration

參考文獻

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