以毛細管電泳技術在二苯甲酮分離之探討

Study on the Analyses of Benzophenone by Capillary Electrophoresis

陳彥蓁(Yen-Chen Chen)

指導教授 ： 黃悉雅

中原大學/理學院/化學研究所/碩士(2004年)

https://doi.org/10.6840/cycu200400293

摘要

摘要

本研究是利用毛細管電泳為分離工具，以微胞電層析(micellar electrokinetic capillary chromatography，MEKC)及微乳化電層析(microemulsion electrokinetic capillary chromatography，MEEKC )兩種不同的方式，對於十種結構相似的二苯甲酮進行分離之研究。而此兩種技術都是利用各分析物親疏水性質在偽靜相及緩衝溶液中滯留時間的不同，達到分離效果。此兩種技術最大的差異在於，前者在緩衝溶液中添加界面活性劑形成微胞，後者是利用油滴、界面活性劑及共界面活性劑形成微乳化。而我們將針對幾個分離因子，如界面活性劑濃度、pH值、溫度、有機修飾劑等探討其對分離效率的影響。
並由實驗結果顯示，在微胞電層析測試時，可藉由改變界面活性劑及溫度來得到最適化分離效果，而微乳化電層析則是以改變pH值及有機修飾劑得到最適化分離效果。而利用微乳化電層析在pH9.0、分離溫度為35℃、在緩衝溶液中添加5％乙醇之最佳條件下成功分離乳液中的防曬劑，並可檢測出其種類及含量。

關鍵字

毛細管電泳技術 ； 微胞電層析與微乳化電層析技術

並列摘要

Abstract

In the study, separation and determination of ten hydrophobic benzophenone which were commonly used as sunscreen additives in various lotions, by micellar electrokinetic chromatography (MEKC) and microemulsion electrokinetic chromatography (MEEKC) were compared. Micelles, which was composed of surfactant monomers, was employed as pseudostationary phases in MEKC. On the other hand, oil droplet, which was stablized by surfactant and co-surfactant, was used pseudostationary phases in MEEKC.
The effect of concentration of surfactant, buffer pH, buffer’s ionic strength, organic solvents, temperature and applied voltage on benzophenone separations were examined. An optimum MEKC method for benzophenone separation was obtained within 9 min with a Tris running buffer of pH 9.2 containing 9.91% (w/w) SDS, 2% (w/w) ethanol. A MEEKC separation with baseline resolution was also obtained within 7 min using a microemulsion of pH 9.2, which composed of 0.6% (w/w) SDS, 1.2% (w/w) 1-butanol, 0.5% (w/w) ethyl acetate, and 5% (w/w) ethanol. Furthermore, the developed MEEKC had a higher separation efficiency than MEKC.

並列關鍵字

MEKC and MEEKC methods ； Capillary Electrophoresis

參考文獻

【24】 王鳳英，界面活性劑的原理與應用，1986。

【2】 Baker D.R, Capillary Electrophoresis, New York, 1995, 21.

【3】 Camilleri P., Capillary Electrophoresis Theory and Practice, Boca Raton, 1998.

【4】 Christian W.K., Klampfl Electrophoresis 2003, 24, 1537.

【8】 Santo Scalia J. of Cromatography A 2000, 870, 199-205.