陳皮萃取物抑制內毒素對巨噬細胞與微神經膠細胞誘導發炎蛋白作用探討

Chenpi Extract Inhibits Endotoxin Induced Inflammatory Protein Activites in Macrophages and Microglias

王一光(Yi-Kuang Wang)

指導教授 ： 何豐名 ； 翁清松

中原大學/工學院/醫學工程研究所/碩士(2006年)

https://doi.org/10.6840/cycu200600416

摘要

無資料

關鍵字

誘導型一氧化碳合成酶 ； 一氧化碳 ； 陳皮萃取物 ； 第二型環氧化酶

並列摘要

Macrophages play a key role in the immune protection system against microorganisms or tumour cells since they partake in the processing of antigens and presentation to lymphocytes, as well as in the phagocytosis and killing of microbes. In this process, high amounts of nitric oxide (NO) and reactive oxygen intermediates are generated contributing to intracellular destructive mechanisms.
While parts of our body are infected with bacteria, macrophages and microglias will secrete cytokines to protect us from being injured. However, if the system secretes too much or too little cytokine, it will cause a threat to cells, tissues, or organs.
Naringenin extract of Chen-pi of Chinese herbal medicine, a kind of antioxidant, is composed of flavanones. Furthermore, flavanones can prevent bacteria and tumors from spreading.
Our study applied naringenin (10-200μM) on cells, and it made an impact on the survival rate of Macrophages and Microglia (30-100μM). In addition, when we treated the macrophages and the microglias with LPS, it stimulated cells to secrete a large amount of nitric oxide. Besides, we treated lipopolysaccharide (LPS) on macrophages and microglias and it stimulated cells to secrete nitric oxide. Also, the study used naringenin to treat the cells and restrained them from being inflamed. According to Western Blot, nitric oxide decreased because naringenin restrained inducible nitric oxide synthase (iNOS) from developing.
In the experiment, our results indicated that the cells would not be inflamed by naringenin. However, after treating LPS stimulation, nitric oxide could be reversed by naringenin. As for the expression of proteins after LPS treatment, the expression of inducible nitric oxide synthase and cyclooxygenase (COX-2) decreased when naringenin was increased. When naringenin was added to the cells, it was able to reduce the amount of iNOS, which diminished the nitric oxide, and COX-2. Therefore, it could be concluded that the cells would not be inflamed or damaged under the proper amount of naringenin.

並列關鍵字

nitric oxide ； COX-2 ； Chen-pi extract ； iNOS