以核定位訊號胜肽鏈結合量子點進行造骨細胞細胞核標定

本研究的目的是將量子點結合核定位訊號胜肽鏈（Nuclear Localization Signal Peptide, NLS Peptide)進行造骨細胞的細胞核標定。在兩性高分子（Poly(maleic anhydride alt-1- tetradecene )）的幫助下，將本實驗所使用的疏水性CdSe/ZnS量子點轉變為親水性。由於量子點需要藉由胺基和核定位訊號結合，所以，本實驗嘗試混合兩種不同結構的聚乙二醇，將表面帶有羧基量點子表面修飾成胺基。經過聚乙二醇修飾後的量子點本身光學及物理特性並未受到影響。進一步探討量子點與聚乙二醇修飾後的穩定性與細胞毒性。結果發現，聚乙二醇修飾後的量子點能夠提高量子點的鹽穩定度，並降低量子點對造骨細胞以及蝕骨細胞的細胞毒性。將聚乙二醇修飾後的量子點和核定位訊號利用Sulfo-SMCC接合後，成功的用來標定造骨細胞的細胞核。本實驗結果未來可以應用在監測成骨細胞細胞核以及前趨蝕骨細胞融合成蝕骨細胞的過程。

關鍵字

蝕骨細胞；造骨細胞；核定位訊號；量子點

並列摘要

The purpose of the study was to conjugate quantum dots with nuclear-localization-signal (NLS) peptide to label osteoblasts’ nuclei. The hydrophobic surface of CdSe/ZnS quantum dots (QDs) was modified to became hydrophilic by Poly(maleic anhydride alt-1-tetradecene). Only QDs with amino groups could be conjugated with NLS Peptide. Hence, the surface with carboxyl group of QDs was modified with amino groups using two different type of poly(ethylene glycol). The result showed that tow kind structure of poly(ethylene glycol) modified QDs (PEG-QDs) did not influence the optical nor the physical characterizations of QDs. The salt stability and cytotoxicity test of QDs and PEG-QDs were investigated. It was found that PEG-QDs were more stable than QDs in with high concentration of NaCl, and had lower cytotoxicity of QDs towards osteoblasts and osteoclasts. The Sulfo-SMCC was used for conjugated PEG-QDs with NLS peptides, and these NLS-PEG-QDs were able to labeled osteoblast nuclei. Successfully, the results of this study could be applied to monitor long-term observation of osteoblast nucleus and cell fusion during osteoclastic formation.

並列關鍵字

Nuclear Localization Signal ； Quantum dot ； Osteoblast

參考文獻

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以核定位訊號胜肽鏈結合量子點進行造骨細胞細胞核標定

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