黑殭菌及黑殭菌素與小皮傘及其代謝物的培養觀察與初步探討

Preliminary study on Cultivation and metabolites of Metarhizium anisopliae and Marasmius oreades

劉芷岑(Jr-Tsen Liou)

指導教授 ： 曾耀銘

朝陽科技大學/理工學院/應用化學系生化科技碩博士班/碩士(2014年)

摘要

真菌的二次代謝產物具有許多生理活性，如抗菌、抗癌、抗發炎、抗真菌。黑殭菌屬(Metarhizium)為昆蟲病原真菌的一種，廣泛使用於世界各地農業害蟲及疾病媒介控制方案中，可替代以往使用的化學殺蟲劑。事實上，環縮酚酸胜肽加上天然環胜肽，成為一個以上酯鍵連結為特色的天然產物大家族，此類天然產物，由於其具有多樣化生物活性，而引起科學界廣大興趣，包含抗癌、抗生素、抗真菌、免疫抑制劑或抗發炎。本實驗將以5L攪拌式發酵槽發酵培養黑殭菌素，並以乙酸乙酯萃取其成分，續以半製備型HPLC分離純化並大量收集，此外，研究顯示小皮傘二次代謝物可抑制孢子萌發，作為抗生素或生物殺蟲劑。本章將探討真菌小皮傘固態培養，並觀察其生長型態，了解小皮傘其他生理活性。

關鍵字

小皮傘 ； 黑殭菌素 ； 黑殭菌

並列摘要

Secondary metabolites of fungi has many physiological activities such as antibacterial, anti-cancer, anti-inflammatory, anti-fungal. Matarhizium is a genus of entomopathogenic funfi used world-wide as alternatives to chemical insecticides in agricultural pest and disease-vector control programs, which can replace chemical pesticides used in the past. In fact, cyclodepsipeptide peptide plus natural peptide ring, becoming a more ester bonds linked featuring a large family of natural products, such natural products, due to their diverse biological activity, which cause scientist interested, because it include anticancer, antibiotic, antifungal, anti-inflammatory and immunosuppressive agents. The destruxin used in this study were isolated and purified from submerged culture,and extracted with ethyl acetate. And used semi-preparative HPLC purification and large collection.Furthermore, before studies show secondary metabolites of Marasmius can inhibit spore germination, it can be antibiotics or biocides. In this chapter we investigate the solid culture of fungus Marasmius , and to observe their growth patterns, understand Marasmius other physiological activity.

並列關鍵字

Marasmius ； Destruxins ； Mtarhizium

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