本實驗以Monascus purpureus BCRC 31615 紅麴菌株,分別以白米、糙米為基質在 30℃ 下進行固態培養紅麴菌,並分析 monacolin K、citrinin 及色素產量。再藉由培養環境的改變,找出其最適的培養條件,以提高 monacolin K 產量。實驗結果發現,以白米培養紅麴時色素產量較高,而以糙米培養紅麴時 monacolin K 產量較高;在糙米的部份,接菌後第九天添加 60 ml 無菌水,monacolin K 產量由原本的 3813 ppm 增加到 5098 ppm,增加了 33.7%。而 monacolin K、citrinin 與色素的生成量皆有相似的趨勢。本研究成果是目前在文獻上所能得到的最佳成果。
In this study, the influence of different rice and cultivation condition on the production of monacolin K, citrinin and pigments by solid state fermentation of Monascus purpureus BCRC 31615 was studied. Monascus was cultured at 30℃ on polished indica rice and de-hulled indica rice, separately. The results showed that the pigment is relatively high when cultivated on polished indica rice while monacolin K content is relatively high when cultivated on de-hulled indica rice. During solid state fermentation of de-hulled indica rice, adding 60 ml of water at day 9 improved 33.7% of the productivity of monacolin K from 3813 ppm to 5098 ppm. A similar tendency was observed in the formation amount of monacolin K, citrinin and pigments. The monacolin K production of this study was the most in the literatures ever published.
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