在利用米根黴 (Rhizopus oryzae ATCC 9363) 生產L型乳酸的過程中,菌絲乾重和幾丁聚醣受尿素添加量的影響。用5公升攪拌式醱酵槽中進行醱酵,當尿素濃度從每公升2.5公克增加到10.0公克,並以氫氧化鈣控制pH值在6.0,菌絲乾重從每公升4.35公克增加到7.25公克,同時幾丁聚醣從0.290公克增加到0.841公克;在尿素濃度每公升5.0公克時,乳酸有最高產量每公升101.3公克。以氫氧化鈉和碳酸氫鈉混合液控制pH值在6.0,添加尿素濃度每公升5.0公克為氮源,在此條件下,以每公升120公克的葡萄糖為受質,菌絲乾重每公升8.15公克,萃取出幾丁聚醣最高為1.362公克。
During L-lactic acid fermentation by culture of Rhizopus oryzae ATCC 9363, the production of biomass and chitosan was affected by the urea concentration. When fermentation was carried out in a 5-L stirred fermenter by adding calcium hydroxide to control the pH at 6.0, biomass increased from 4.35 g/L to 7.25 g/L as urea from 2.5 to 10.0 g/L was used as N-source; meanwhile, the produced chitosan increased from 0.290 g to 0.841 g. The highest yield of L-lactic acid was 101.3 g/L as 5.0 g/L of urea was used. Upon 5.0 g/L of urea as N-source and 120 g/L of glucose as substrate, 8.15 g of biomass and the highest yield of chitosan at 1.362 g were obtained when pH was controlled at 6.0 by adding a mixture of sodium hydroxide and sodium bicarbonate.
為了持續優化網站功能與使用者體驗,本網站將Cookies分析技術用於網站營運、分析和個人化服務之目的。
若您繼續瀏覽本網站,即表示您同意本網站使用Cookies。