火炭母草及重陽木甲醇萃取物之抗發炎及抗癌活性研究

Anti-inflammatory and Antitumor Activities of Methanolic Extracts of Polygonum chinensis and Bischofia javanica

林坤源(Kun-Yuan Lin)

指導教授 ： 李綉鈴

大同大學/工程學院/生物工程學系所/碩士(2009年)

摘要

體外試驗發現火炭母草及重陽木之甲醇萃取物具有很好的抗氧化活性。本研究以動物試驗探討火炭母草及重陽木甲醇萃取物的抗發炎能力及對人類癌細胞生長之影響。結果顯示將此二植物甲醇萃取物局部塗抺於小鼠耳朵時，3 mg/ear可顯著的抑制PMA (phorbol myristate acetate)誘發之急性發炎作用；而以oxazolone誘發過敏性接觸皮膚炎的模式下，火炭母草甲醇萃取物在3 mg/ear的塗抺濃度，明顯的抑制小鼠耳朵的過敏性發炎腫脹 (p＜0.05)，而重陽木甲醇萃取物在1及3 mg/ear的塗抺劑量則均無抑制之現象；小鼠口服火炭母草及重陽木甲醇萃取物之最大容忍劑量250 mg/kg後，其結果並無顯著地抑制λ-carrageenan誘發小鼠脚掌之急性發炎腫脹。此外，火炭母草及重陽木甲醇萃取物可顯著的抑制第二型環氧化酶活性，其IC50值分別為11 及8 mg/ml，但對第一型環氧化酶則表現出較差之抑制作用，其IC50值分別為97 及180 mg/ml。
在抗腫瘤方面，火炭母草及重陽木的甲醇萃取物在100及1000 mg/ml之濃度下，均顯著地抑制五種人類癌細胞株的細胞生長，包括人類乳癌細胞株(T47D)、人類大腸癌細胞株 (HT-29)、人類肺癌細胞株 (A549)、人類胰臟癌細胞株 (PANC-1)及人類前列腺癌細胞株 (PC-3) 。在抑制血管新生的體外試驗中，火炭母草及重陽木之甲醇萃取物在100及1000 mg/ml二個濃度時，雖然顯著的抑制人類臍帶靜脈內皮細胞形成微管，但在10及100 mg/ml濃度下，則對人類臍帶靜脈內皮細胞(HUVEC)具有毒殺作用。

關鍵字

抗血管新生 ； 抗癌 ； 火炭母草 ； 重陽木 ； 抗發炎 ； 甲醇萃取物

並列摘要

The methanolic extracts of Polygonum chinensis and Bischofia javanica exhibit strong antioxidant activity. The anti-inflammatory and antitumor activities of methanolic extracts from these two herbal medicines were conducted in this study. These results revealed that the methanolic extracts of P. chinensis and B. javanica at 3 mg/ear exhibited significant inhibition of acute ear edema induced by phorbol myristate acetate (PMA) in mice. In addition, the methanolic extract of P. chinensis at 3 mg/ear showed a significant decrease of mouse ear swelling in oxazolone induced allergic dermatitis. while B. javanica at doses of 1 and 3 mg/ear did The two methanolic extracts administered by oral gavage at 250 mg/kg did not elicit any significant inhibition by λ- carrageenan induced paw edema in mice. The methanolic extracts of P. chinensis and B. javanica exhibited stronger inhibitory activities of cyclooxygenase (COX)-2 than COX-1. The IC50 value of P. chinensis and B. javanica were 11 and 8 mg/ml for COX-2 as well as 97 and 180 mg/ml for COX-1, respectively. At concentrations of 100 and 1000 mg/ml, these two extracts both showed significant inhibition of tumor cell proliferation for T47D (breast), HT-29 (colon), A549 (lung), PANC-1 (pancreas) and PC-3 (prostate). In anti-angiogenesis assay, microtube formation from human umbilical vein endothelial cells (HUVEC) was also found at 100 and 1000 mg/ml. However, P. chinensis and B. javanica at 10 and 100 mg/ml exhibited significant cytotoxicity in the growth of HUVEC.

並列關鍵字

Anti-angiogenesis ； Antitumor ； Polygonum chinensis ； Bischofia javanica ； Anti-inflammatory ； Methanolic Extracts

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