培養環境對細本山葡萄（Vitis thunbergii Sieb. et Zucc.）細胞生產二次代謝物之影響

The effect of culture condition on the production of secondary metabolites in cell culture of Vitis thubergii sieb. et zucc.

朱培昇(Pei-Sheng Chu)

指導教授 ： 何錦玟

大同大學/工程學院/生物工程學系所/碩士(2009年)

摘要

七年長期繼代之細本山葡萄（Vitis thunbergii Sieb. et Zucc.）癒合組織（long-term callus），以固態培養21天後，細胞可增加至接種量的8倍，細胞內resveratrol總含量為50.8 mg/kg DW。藉由改變環境因子刺激癒合組織內resveratrol之累積，在21天的培養週期中，癒合組織經一週的培養後通以0.5 V之直流電一天，其resveratrol可達到265.0 ± 50.8 mg/kg DW，為控制組104.2 ± 26.4 mg/kg DW的2-3倍。在植物栽培燈及綠色、紅色、藍色 LED燈的四種不同光源環境下，細胞鮮重以綠光中的生長量最佳，可由1 g 增生至7.55 g ；在resveratrol總含量及總酚量方面，均以培養於植物栽培燈下有最高的含量，分別為106.6 mg/kg DW及4947 eq. catechin mg/kg DW。在UV光的照射方面，僅在第一天以UV光照射10或30分鐘，resveratrol總含量略為增加（177.2 及139.2 mg/kg DW），其他處理對細胞之生長有促進作用，但對resveratrol之累積皆有抑制作用。在異種細胞共同培養中，在總酚含量上，當細本山葡萄細胞與白花蛇舌草之接種比例為2：1時，總酚含量可由9923.3 eq. catechin mg/kg DW提升至15407.2 eq. catechin mg/kg DW。

關鍵字

組織培養 ； 細本山葡萄 ； 白藜蘆醇

並列摘要

The biomass of long-term cell which were cultured for seven years increased nearly 8 times within 3 weeks cultured on 1/2 MS solid medium supplemented with 1.86 mgl-1 NAA and 0.22 mgl-1 BA and the total resveratrol content was 50.8 mg/kg DW. The total resveratrol content reached to 265.0 ± 50.8 mg/kg DW, which was two times to the control（without electric current, 104.2 ± 26.4 mg/kg DW）,when treat with 0.5V electric current for one day after cultured one week. Cells were cultured with different light sources（florescence, green LED, red LED and blue LED）, the growth index were the highest（6.55）when cultured under the green light. When cultured under florescence light, the total resveratrol content and total phenol were the best, 106.6 mg/kg DW and 4947 eq. catechin mg/kg DW, respectively. Cells treated with UV for 10 or 30 minutes at the first day of culture increased the content of total resveratrol（177.2 and 139.2 mg/kg DW）and didn’t effect the growth of biomass. In the co-culture system, the total phenol in cell was increased from 9923.3 to 15407.2 eq. catechin mg/kg DW, when the ratio of Vitis thunbergii cells and Hedyotis diffusa cells were 2：1.

並列關鍵字

Plant cell culture ； Vitis thunbergii ； resveratrol

參考文獻

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