馬兜鈴酸液相層析分析及中藥材毒性分類探討

Survey of the Occurrence of Aristolochic Acid in Fangchi obtained from Mainland China by HPLC and The classification of the toxicity of traditional Chinese medicines

游尚航(Shang Hang YU)

指導教授 ： 鄧正賢

亞洲大學/健康學院/生物科技學系碩士班/碩士(2009年)

摘要

本研究之目的在於調查大陸地區在禁用馬兜鈴酸藥材後的現狀調查。於2006年收集大陸各省份地區防己藥材共三十四家，以 4℃以下真空冷藏，並將樣品於前處理竭盡萃取完後入液相層析。

分析方法流程為取 1.0 g 樣品粉碎加入 200 mL甲醇溶媒，超音波震盪 30 分鐘，濾紙過濾，濃縮定量至10 mL。高效液相層析儀分析馬兜鈴酸指標成分時，移動相為CH3CN / H2O / CH3COOH (45：53：2, v/v)，流速為0.8mL/min，檢測螢光波長為 254 nm。利用上述分析技術進行對照標準品馬兜鈴酸分析，結果顯示六個樣品含有馬兜鈴酸。得線性迴歸方程式(Y=mX+b)及相關係數(r)均顯示良好線性關係。同日內相對標準偏差及回收率均在可接受範圍。

關鍵字

馬兜鈴酸 ； 中藥材毒性分類探討 ； 液相層析

並列摘要

The purpose of this study was to investigate the present situation of the presence of Aristolochic Acid (AA) in Fangchi or Fangji crude drugs in China after issue of a warning on AA in November 1999. All Fangchi materials were purchased from the pharmacies of Traditional Chinese Medicines in Mainland China in 2006, and a total of twelve samples were collected. All samples arrived at our laboratory frozen and were stored at ≦ 4℃ until analysed. All extracts of samples were analysed on the same day as they were prepared.

The samples were analyzed by HPLC on a Thermo Electron Corporation C12 column and detected at 254 nm with acetonitrile water and 2% (v/v) acetic acid (45:55, v/v) as the mobile phase at a flow rate of 0.8 ml/min. 34 samples of commericial fangchi were analyzed and the results showed that 6 samples contained aristolochic acid I and aristolochic acid II . The content of aristolochic acid I and aristolochic acid II in sample .

並列關鍵字

Aristolochic Acid ； HPLC ； traditional Chinese medicines

參考文獻

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參考文獻