刀傷草甲醇萃取物之抗氧化活性與其抗氧化成份研究

Studies on Antioxidative Activity and Antioxidant Components of Methanolic Extracts from Ixeris laevigata

張立嘉(Li Ja Chang)

指導教授 ： 蔡政芳

亞洲大學/健康學院/生物科技學系碩士班/碩士(2009年)

摘要

刀傷草(Ixeris laevigata)為菊科(Compositae)苦蕒菜屬(Ixeris)植物。其為臺灣普遍之民間用藥，可用以止痛、止血、消腫、消炎及退燒等功效。但在目前文獻中，刀傷草之相關研究較為有限，故藉由研究刀傷草甲醇萃取物之還原力、螯合亞鐵離子能力、DPPH自由基清除能力、抗氧化力與刀傷草甲醇萃取物之天然抗氧化成份含量，包括多酚類、黃酮類、抗壞血酸、β-胡蘿蔔素及生育醇等，以探討其抗氧化活性。
根據實驗結果，100％刀傷草甲醇萃取物之還原力和DPPH自由基清除能力均比50％刀傷草甲醇萃取物強；而50％和100％刀傷草甲醇萃取物之螯合亞鐵離子能力則不及EDTA。對於50％和100％刀傷草甲醇萃取物所做之抗氧化試驗，50％、100％刀傷草甲醇萃取物與BHT實驗組均有吸光值降低的情況;另外在抗氧化成分分析部份，100%與50%刀傷草甲醇萃取物分別有130.77與87.96mg/g的含量，在黃酮類的部份發現這兩者分別有26.64與13.43 mg/g的含量。整體而言就抗氧化活性與抗氧化成份兩者均有其正相關性，故刀傷草是具有應用潛力之天然抗氧化劑。

關鍵字

刀傷草 ； 抗氧化活性 ； 抗氧化成份

並列摘要

Ixeris laevigat (IL), Family Compositae, , is a folk medicine, used in Taiwan, it also called Tao-Shaong-Ts’ao. This plant is commonly used for the treatment of bleeding, swelling, inflammation, fever and pain. Furthermore, it has been used to treat hepatitis, pneumonia, diarrhea and anti-liver fibrosis, but there is no scientific study available for its usefulness in antioxidant activities. To understand the effects of antioxidant of IL extracts, we used several experiment methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect, reducing power, ferrous chelating ability, and antioxidant activities were investigated. Not only antioxidant activities, but also antioxidant components, including total phenols, flavonoids, ascorbic acids, tocopherols, and carotenoids. The results showed high antioxidant activities. Scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, it was found higher radical-scavenging activity, 100% MeOH extract of IL possessed a little better than 50% MeOH extract of IL. The DPPH scavenging effects of 50% and 100% MeOH extract of IL were 93.5% and 95% at 0.3mg/ml, respectively. The reducing powers of 50% and 100% MeOH extract of IL were 0.74 and 1.0 at 0.4mg/ml, respectively. The results showed that the 100% MeOH extract of IL possessed an excellent reducing power, but in the ferrous chelating ability test, it was found the chelating ability of these two IL extract’s were lower than EDTA in this test. To understand why the IL extracts have such an good effects in antioxidant activities that we do some compound analysis. Total phenols were the antioxidant components found in two extracts and in the range of 87.96–130.77 mg/g. Flavonoid also found in two extracts and in the range of 26.64–13.43 mg/g. In conclusion, based on the results obtained, these extracts from IL were effective in antioxidant activities.

並列關鍵字

Ixeris laevigata ； Antioxidative Activity ； Antioxidant Components

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