Involvement of Polyamines in the Contrasting Sensitivity of Chickpea (Cicer arietinum L.) and Soybean (Glycine max (L.) Merrill.) to Water Deficit Stress

多胺涉及鴨豆及大豆對缺水之靈敏度

Harsh Nayyar ； Satwinder Kaur ； Smita ； Sanjeev Kumar ； K. J. Singh ； K. K. Dhir

《Botanical Bulletin of Academia Sinica》 46卷4期 (2005/10) Pp. 333-338

https://doi.org/10.7016/BBAS.200510.0333

摘要

使鴨豆(Cicer arietinum L. cv. GPF2)及大豆(Glycine max (L.) Merrill cv. Brag)處於不同程度之缺水逆境（-0.2到-0.8MPa）以評估它們對缺水之不同靈敏度。大豆顯然較鴨豆易受缺水傷害（在所有供試情形下，以電解質之流失作判斷）依發芽百分率，根長度及根生長率為測定項目所定出之LD50（達50%時之劑量）在大豆為-0.4MPa而鴨豆為-0.6MPa（表示後者較耐缺水逆境）。當處於缺水逆境時，鴨豆根部之水含量較大豆高。以發芽後七天之細菌為例，當處於-0.8MPa時，根部之多胺（個別或總量）的增加鴨豆顯著地高於大豆。不論那一種豆類，當內在之多胺含量下降時，受缺水之傷害會加重。大豆根部，相對於鴨豆根部，多胺之減少（尤其是putrescine及spermidime）和它的較易受缺水傷害及較少之水含量息息相關。外加之putrescine及spermidine大大地減緩缺水造成之傷害（尤其是大豆較明顯）。分別以putrescine及spermidime之生合成抑制劑做試驗証實了上述觀點。

關鍵字

Cicer arietinum ； Glycine max ； 多胺 ； Putrescine ； Spermidine ； Spermine ； 缺水逆境

並列摘要

Chickpea (Cicer arietinum L. cv. GPF2) and soybean (Glycine max (L.) Merrill cv. Brag) genotypes were subjected to varying water stress levels of -0.2 to -0.8 MPa to assess their relative sensitivity towards water stress. Soybean seedlings experienced significantly more stress injury (as electrolyte leakage) than chickpea at all stress levels. LD50 in terms of percent germination, root length, and root growth rate occurred at -0.4 MPa in soybean and at -0.6 MPa in chickpea. In chickpea, the root water content was higher than in soybean during stress. Endogenous levels of individual as well as total polyamines (PAs) in roots of 7-d-old seedlings subjected to -0.8 MPa stress increased to a significantly greater extent in chickpea than in soybean. The stress injury was accentuated as the PA levels declined in both the plant types. The reduced levels of PAs in soybean, especially putrescine (PUT) and spermidine (SPD) relative to chickpea, were related to higher stress injury and decreased water content. Exogenous PUT and SPD markedly mitigated the stress-induced effects, particularly in soybean. Inhibitor studies involving α-Difluromethylarginine (DFMA) and α-difluromethylornithine (DFMO), the biosynthetic inhibitors of PUT, as well as cyclohexylamine (CHA), biosynthetic inhibitor of SPD and SPM. corroborated the role of PAs in mediating the differential sensitivity of chickpea and soybean to water stress.

並列關鍵字

Cicer arietinum ； Glycine max ； Polyamines ； Putrescine ； Spermidine ； Spermine ； Water stress.