Resistant starch is one of the index components for dietary fiber. Analysis of variance for the effects of crop seasons and varieties (lines) on the resistant starch content of fresh tuberous roots of sweet potato indicated that these two factors interacted significantly. A significant difference in contents of resistant starch was found among varieties (lines), and sweet potato grown in Spring crop had higher value than that grown in Fall crop. The correlation coefficient of resistant starch and amylose content was higher in Spring crop. The resistant starch content in fresh tuberous roots of sweet potato was not significantly changed due to different storage periods. In order to conducive to breeding and storage process, the varieties (lines) of sweet potato had a higher resistant starch content in tuberous roots and a good quality during storage period will be a better choice.