- 期刊
以超高效能液相層析法快速同時定量分析白芷7種香豆素成分及美白活性之評估
Simultaneous Quantification of Seven Coumarins in Angelica dahurica (Bai Zhi) Using Ultra Performance Liquid Chromatography and Its Whitening Efficiency Assessment
摘要
本研究建立同時分析7 種香豆素的快速方法，並探討白芷栽種時期各部位之香豆素含量變化和抑制酪胺酸酶活性。超高效能液相層析儀 (UPLC) 使用管柱為ethylene bridged hybrid (BEH) C_(18)，在酸化水 (pH 3.0) 沖提下，改變氰甲烷 (acetoniltrile) 濃度由15% 至95% 使成梯度沖提，可在12 min 之內，同時有效率的分離並定量香豆素的含量。分析結果顯示，呋喃香豆素，包括psoralen、xanthotoxin、bergapten 及8-hydroxybergapte等成分皆可在種植9 mo 的白芷根部檢測其含量，其中8-hydroxybergapten 含量皆高過其他呋喃香豆素10 餘倍。種植3 mo 及6 mo 之白芷莖部抑制酪胺酸酶能力為最佳。高抑制酪胺酸酶之白芷莖部香豆素，未來可進行萃取並應用於化妝品工業。
並列摘要
An improved ultra performance liquid chromatography method was developed to simultaneously quantify seven coumarins isolated from various organs of Bai Zhi [Angelica dahurica (Fish.) BENTH. et HOOK]. An efficient separation and quantification of coumarin can be established within 12 min using ethylene bridged hybrid (BEH) C_(18) column and a gradient elution system with a acetonitrile concentrations varying from 15 to 95% (v/v) in acidic water (pH 3.0) elution. The furanocoumarins such as psoralen, xanthotoxin, bergapten, and 8-hydroxybergapten were detected in 9 month-old Bai Zhi roots, in which the content of 8-hydroxybergapten was 10-fold higher than other coumarins. Nevertheless, stems from 3 and 6 month-old Bai Zhi exhibited the highest inhibitory ability on tyrosinase activity. In the future, coumarins extracted from the stems of Bai Zhi with high inhibitory effect on tyrosinase could be further used in the cosmetic industry.
延伸閱讀
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