內生真菌Lasmenia sp. CB10菌株培養基質之篩選暨評估其防治番茄萎凋病之潛力

Screening the Culture Substrates for Endophytic Fungi, Lasmenia sp. CB10, and Evaluation of Its Effect on Control of Tomato Fusarium wilt

羅佩昕(Pei-Hsin Lo) ； 鍾文全(Wen-Chuan Chung) ； 鍾文鑫(Wen-Hsin Chung)

《台灣農業研究》 66卷3期 (2017/09) Pp. 193-201

https://doi.org/10.6156/JTAR/2017.06603.03

摘要

本研究將分離自佛手柑之內生真菌Lasmenia sp. CB10菌株培養於不同基質，證實CB10菌株培養於米糠(rice bran)之基質(簡稱RBCB10)對抑制番茄萎凋病菌Fol146菌株菌絲生長效果最佳。測試於栽培介質混合不同比例之RBCB10對番茄植株生長影響，結果指出混合0.5% (w/v) 或1% (w/v)之RBCB10皆不會影響番茄生長。進一步將0.5% (w/v) RBCB10與帶Fol146菌株之感染土混合，證實添加RBCB10可減少Fol146菌株之族群量。另比較RBCB10基質之不同施用方式對其防治番茄萎凋病之效果，得知以混合0.5% (w/v) RBCB10之泥炭土作為育苗介質，可降低萎凋病的罹病度達26.7%；而直接將0.5% (w/v) RBCB10添加於感染土之處理，則可降低萎凋病罹病度達40.7%。未來於田間試驗時可直接以添加方式將RBCB10混合於土壤中，以達延緩番茄萎凋病之效果。

關鍵字

內生真菌 ； Lasmenia sp. ； 番茄萎凋病 ； 米糠

並列摘要

The endophytic fungi Lasmenia sp. CB10 was isolated from medicinal plant, fingered citron (Citrus medica var. sarcodactylis). The screening test indicated that CB10 grew in rice bran (RBCB10) showed highly efficacy on inhibiting the mycelium growth of Fusarium oxysporum f. sp. lycopersici (Fol146). Moreover, peat moss mixed with 0.5% or 1% (w/v) RBCB10 did not promote the growth of tomato seedlings significantly. The control efficacy demonstrated that the infested soil of Fol146 mixed with 0.5% (w/v) RBCB10 could decrease the population of Fol146. Compared with different application method of RBCB10, the peat moss mixed with 0.5% (w/v) RBCB10 used as seedbed soil then the tomato seedlings were transplanted into infested soil of Fol146 could decrease 26.7% disease severity of tomato Fusarium wilt. On the other hand, the infested soil of Fol146 mixed with 0.5% (w/ v) RBCB10 directly could decrease the disease severity by 40.7%. The results suggested that field soil mixed with RBCB10 directly might be suitable for delaying the severity of tomato Fusarium wilt under field condition.

並列關鍵字

Endophytic fungi ； Lasmenia sp. ； Fusarium wilt ； Rice bran